|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM298441276 |
| 003 |
DE-627 |
| 005 |
20231225094149.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2019 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.jplph.2019.03.009
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0994.xml
|
| 035 |
|
|
|a (DE-627)NLM298441276
|
| 035 |
|
|
|a (NLM)31229903
|
| 035 |
|
|
|a (PII)S0176-1617(18)30651-5
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Vega-Mas, I
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Tomato roots exhibit in vivo glutamate dehydrogenase aminating capacity in response to excess ammonium supply
|
| 264 |
|
1 |
|c 2019
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 06.08.2019
|
| 500 |
|
|
|a Date Revised 07.12.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2019 Elsevier GmbH. All rights reserved.
|
| 520 |
|
|
|a In higher plants ammonium (NH4+) assimilation occurs mainly through the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway. Nevertheless, when plants are exposed to stress conditions, such as excess of ammonium, the contribution of alternative routes of ammonium assimilation such as glutamate dehydrogenase (GDH) and asparagine synthetase (AS) activities might serve as detoxification mechanisms. In this work, the in vivo functions of these pathways were studied after supplying an excess of ammonium to tomato (Solanum lycopersicum L. cv. Agora Hybrid F1) roots previously adapted to grow under either nitrate or ammonium nutrition. The short-term incorporation of labelled ammonium (15NH4+) into the main amino acids was determined by GC-MS in the presence or absence of methionine sulphoximine (MSX) and azaserine (AZA), inhibitors of GS and GOGAT activities, respectively. Tomato roots were able to respond rapidly to excess ammonium by enhancing ammonium assimilation regardless of the previous nutritional regime to which the plant was adapted to grow. The assimilation of 15NH4+ could take place through pathways other than GS/GOGAT, since the inhibition of GS and GOGAT did not completely impede the incorporation of the labelled nitrogen into major amino acids. The in vivo formation of Asn by AS was shown to be exclusively Gln-dependent since the root was unable to incorporate 15NH4+ directly into Asn. On the other hand, an in vivo aminating capacity was revealed for GDH, since newly labelled Glu synthesis occurred even when GS and/or GOGAT activities were inhibited. The aminating GDH activity in tomato roots responded to an excess ammonium supply independently of the previous nutritional regime to which the plant had been subjected
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Ammonium nutrition
|
| 650 |
|
4 |
|a Asparagine synthetase
|
| 650 |
|
4 |
|a Azaserine
|
| 650 |
|
4 |
|a Glutamate dehydrogenase
|
| 650 |
|
4 |
|a Isotope labelling
|
| 650 |
|
4 |
|a Methionine sulphoximine
|
| 650 |
|
7 |
|a Ammonium Compounds
|2 NLM
|
| 650 |
|
7 |
|a Fertilizers
|2 NLM
|
| 650 |
|
7 |
|a Glutamate Dehydrogenase
|2 NLM
|
| 650 |
|
7 |
|a EC 1.4.1.2
|2 NLM
|
| 700 |
1 |
|
|a Rossi, M T
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Gupta, K J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a González-Murua, C
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ratcliffe, R G
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Estavillo, J M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a González-Moro, M B
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 239(2019) vom: 05. Aug., Seite 83-91
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
|
| 773 |
1 |
8 |
|g volume:239
|g year:2019
|g day:05
|g month:08
|g pages:83-91
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.jplph.2019.03.009
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 239
|j 2019
|b 05
|c 08
|h 83-91
|