iTRAQ protein profile analysis of young and old leaves of cotton (Gossypium hirsutum L.) differing in response to alkali stress

Copyright © 2019 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 141(2019) vom: 15. Aug., Seite 370-379
1. Verfasser: Hu, Yongjun (VerfasserIn)
Weitere Verfasser: Zhao, Long, Zhou, Ji, Zhong, Xiuli, Gu, Fengxue, Liu, Qi, Li, Haoru, Guo, Rui
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Alkali stress Gossypium hirsutum L Old leaves Proteomics Young leaves iTRAQ Alkalies Peptides Plant Proteins mehr... Salts Chlorophyll 1406-65-1 Trypsin EC 3.4.21.4 Nitrogen N762921K75
Beschreibung
Zusammenfassung:Copyright © 2019 Elsevier Masson SAS. All rights reserved.
Proteins will provide a new perspective and deeper understanding for the research of crop alkali tolerance. The aims of this study were to determine and identify the differentially abundant proteins and adaptive mechanisms to alkali tolerance between young and old leaves of cotton. The 4704 proteins were identified, in which 1490 were significantly changed in young leaves and 563 were changed in old leaves in response to alkali stress. The differentially abundant proteins were classified into 10 functional categories in the young leaves, and only 3 functional categories were involved in the old leaves. In the photoreaction system, the accumulations of differential proteins, especially Psb proteins, were higher in young leaves than in old leaves. Compared with old leaves, the carbon metabolism was enhanced significantly through an increased chlorophyll content and increased expression of key proteins for carbon metabolism in young leaves. Furthermore, alkali stress revealed more complex effects on the nitrogen metabolism in young leaves than that in old leaves. Our results demonstrated that during adaptation of cotton to alkali stress, young and old leaves have distinct mechanisms of molecular metabolism regulation. The metabolic flexibility was more remarkable in young leaves than in old leaves; therefore, the alkali tolerance of young leaves is more efficient. These data will increase our understanding of alkali-tolerant mechanisms in higher plants
Beschreibung:Date Completed 05.08.2019
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2019.06.019