Detection and Characterization of Fusarium oxysporum f. sp. vasinfectum VCG0114 (Race 4) Isolates of Diverse Geographic Origins

A highly virulent cotton wilt pathogen, Fusarium oxysporum f. sp. vasinfectum VCG0114 (race 4) was found in West Texas in 2017, after being known in California since 2001. Isolates obtained from wilted plants collected in 2017 from Texas, in 2015 from China, and during 2001 to 2014 from California a...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Plant disease. - 1997. - 103(2019), 8 vom: 10. Aug., Seite 1998-2009
1. Verfasser: Bell, Alois A (VerfasserIn)
Weitere Verfasser: Gu, Aixing, Olvey, Jim, Wagner, Tanya A, Tashpulatov, Javlon J, Prom, Sandria, Quintana, Jose, Nichols, Robert L, Liu, Jinggao
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article f. sp. Fusarium wilt Pima VCG0114 cotton pathogen detection pathogen diversity race 4 DNA, Fungal
LEADER 01000naa a22002652 4500
001 NLM298056615
003 DE-627
005 20231225093333.0
007 cr uuu---uuuuu
008 231225s2019 xx |||||o 00| ||eng c
024 7 |a 10.1094/PDIS-09-18-1624-RE  |2 doi 
028 5 2 |a pubmed24n0993.xml 
035 |a (DE-627)NLM298056615 
035 |a (NLM)31188737 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Bell, Alois A  |e verfasserin  |4 aut 
245 1 0 |a Detection and Characterization of Fusarium oxysporum f. sp. vasinfectum VCG0114 (Race 4) Isolates of Diverse Geographic Origins 
264 1 |c 2019 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 09.08.2019 
500 |a Date Revised 09.08.2019 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a A highly virulent cotton wilt pathogen, Fusarium oxysporum f. sp. vasinfectum VCG0114 (race 4) was found in West Texas in 2017, after being known in California since 2001. Isolates obtained from wilted plants collected in 2017 from Texas, in 2015 from China, and during 2001 to 2014 from California and isolates from historical collections including the race 4 reference isolate were characterized by soil-infestation pathogenicity assays, DNA sequence analysis, and vegetative compatibility analysis. All obtained F. oxysporum f. sp. vasinfectum isolates belonged to VCG0114. All of these isolates, except one isolate from China, caused disease in a soil-infestation assay without nematodes. Thus, they belong to the nematode-independent pathotype. Texas isolates were significantly more virulent than were isolates from China or California on Gossypium barbadense 'Pima S-7'. Four different genotypes (N, T, MT, and MiT) were identified based on the transposable element Tfo1 insertion into the PHO gene and independent MULE or MITE insertions into the Tfo1 transposon. Some significant differences in virulence were detected among the genotypes in some locations. No differences in pathogenicity were observed between the California and China collection isolates on Pima S-7, and the virulence of the major genotypes was similar on the Gossypium hirsutum cultivar 'Stoneville 474' or the Barbren 713 germplasm line. Simple polymerase chain reaction (PCR) methods were developed to specifically determine and detect the four genotypes within VCG0114. A specific PCR method to detect all VCG0114 isolates was also developed. These methods will facilitate the timely identification of infested fields and seed lots and the elucidation of evolutionary relationships among the isolates. This should help to closely monitor the movement of the pathogen and reduce dissemination of these devastating pathogens 
650 4 |a Journal Article 
650 4 |a f. sp. 
650 4 |a Fusarium wilt 
650 4 |a Pima 
650 4 |a VCG0114 
650 4 |a cotton 
650 4 |a pathogen detection 
650 4 |a pathogen diversity 
650 4 |a race 4 
650 7 |a DNA, Fungal  |2 NLM 
700 1 |a Gu, Aixing  |e verfasserin  |4 aut 
700 1 |a Olvey, Jim  |e verfasserin  |4 aut 
700 1 |a Wagner, Tanya A  |e verfasserin  |4 aut 
700 1 |a Tashpulatov, Javlon J  |e verfasserin  |4 aut 
700 1 |a Prom, Sandria  |e verfasserin  |4 aut 
700 1 |a Quintana, Jose  |e verfasserin  |4 aut 
700 1 |a Nichols, Robert L  |e verfasserin  |4 aut 
700 1 |a Liu, Jinggao  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant disease  |d 1997  |g 103(2019), 8 vom: 10. Aug., Seite 1998-2009  |w (DE-627)NLM098181742  |x 0191-2917  |7 nnns 
773 1 8 |g volume:103  |g year:2019  |g number:8  |g day:10  |g month:08  |g pages:1998-2009 
856 4 0 |u http://dx.doi.org/10.1094/PDIS-09-18-1624-RE  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 103  |j 2019  |e 8  |b 10  |c 08  |h 1998-2009