Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site

Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis methods. A small backbone also opens up for add...

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Veröffentlicht in:	BioTechniques. - 1991. - 66(2019), 6 vom: 01. Juni, Seite 254-259
1. Verfasser:	Staal, Jens (VerfasserIn)
Weitere Verfasser:	Alci, Kübra, De Schamphelaire, Wouter, Vanhoucke, Martine, Beyaert, Rudi
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2019
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article PCR cloning minimalism mutagenesis plasmids recombination repository synthetic biology synthetic nucleotide