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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b04018
|2 doi
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|a eng
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|a Verma, Indu
|e verfasserin
|4 aut
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|a Liquid Crystal based Detection of Pb(II) Ions Using Spinach RNA as Recognition Probe
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|c 2019
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|a Text
|b txt
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 17.08.2020
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|a Date Revised 17.08.2020
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|a published: Print-Electronic
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|a ErratumIn: Langmuir. 2019 Jul 30;35(30):9994. doi: 10.1021/acs.langmuir.9b02053. - PMID 31322354
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|a Citation Status MEDLINE
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|a We report a new method for label-free, sensitive, and facile detection of lead(II) ions (Pb2+) based on an aptamer-target binding event, which is recognized by orientations of liquid crystals (LCs) at aqueous interfaces. The LC film suspended in the aqueous phase demonstrated a homeotropic orientation in contact with a cationic surfactant cetyltrimethylammonium bromide (CTAB) due to self-assembly of CTAB molecules at the aqueous-LC interface. The ordering of LC subsequently changed to planar in the presence of the spinach RNA aptamer (SRNA) due to interactions between CTAB and SRNA. In the presence of the Pb2+ ion, the ordering of LC changed to homeotropic caused by reorganization of CTAB at the LC-aqueous interface. This is due to formation of more stable quadruplex structures of SRNA with Pb2+ ions in comparison to the CTAB-SRNA complex. The sensor exhibited a detection limit of 3 nM, which is well below the permissible limit of Pb2+ in drinking water. Our experiments establish that addition of Pb2+ leads to (i) the formation of Pb2+-SRNA complexes and (ii) a decrease in density of SRNA on the LC interface, but additional studies are required to determine which of these processes underlie the response of the LCs to the Pb2+. We have also demonstrated the potential application of the LC sensor for detection of Pb2+ in tap water. Unlike current laboratory-based heavy-metal-ion assays, this method is comparatively simple in terms of instrumentation, operation, and optical readout
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Molecular Probes
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|a RNA, Plant
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|a Lead
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|a 2P299V784P
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|a Devi, Manisha
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|4 aut
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|a Sharma, Diksha
|e verfasserin
|4 aut
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|a Nandi, Rajib
|e verfasserin
|4 aut
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|a Pal, Santanu Kumar
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
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|g 35(2019), 24 vom: 18. Juni, Seite 7816-7823
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|g year:2019
|g number:24
|g day:18
|g month:06
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|u http://dx.doi.org/10.1021/acs.langmuir.8b04018
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