The mitogen-activated protein kinase 4-phosphorylated heat shock factor A4A regulates responses to combined salt and heat stresses

© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 70(2019), 18 vom: 24. Sept., Seite 4903-4918
1. Verfasser: Andrási, Norbert (VerfasserIn)
Weitere Verfasser: Rigó, Gábor, Zsigmond, Laura, Pérez-Salamó, Imma, Papdi, Csaba, Klement, Eva, Pettkó-Szandtner, Aladár, Baba, Abu Imran, Ayaydin, Ferhan, Dasari, Ramakrishna, Cséplő, Ágnes, Szabados, László
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis MAP kinases combined stress heat heat shock factor A4A phosphorylation promoter binding salinity mehr... transcription regulation Arabidopsis Proteins HSFA4A protein, Arabidopsis Transcription Factors Sodium Chloride 451W47IQ8X AtMPK4 protein, Arabidopsis EC 2.7.11.24 Mitogen-Activated Protein Kinases
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100 1 |a Andrási, Norbert  |e verfasserin  |4 aut 
245 1 4 |a The mitogen-activated protein kinase 4-phosphorylated heat shock factor A4A regulates responses to combined salt and heat stresses 
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500 |a Date Completed 27.07.2020 
500 |a Date Revised 28.06.2021 
500 |a published: Print 
500 |a ErratumIn: J Exp Bot. 2021 Jul 28;72(15):5781. - PMID 34180987 
500 |a Citation Status MEDLINE 
520 |a © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. 
520 |a Heat shock factors regulate responses to high temperature, salinity, water deprivation, or heavy metals. Their function in combinations of stresses is, however, not known. Arabidopsis HEAT SHOCK FACTOR A4A (HSFA4A) was previously reported to regulate responses to salt and oxidative stresses. Here we show, that the HSFA4A gene is induced by salt, elevated temperature, and a combination of these conditions. Fast translocation of HSFA4A tagged with yellow fluorescent protein from cytosol to nuclei takes place in salt-treated cells. HSFA4A can be phosphorylated not only by mitogen-activated protein (MAP) kinases MPK3 and MPK6 but also by MPK4, and Ser309 is the dominant MAP kinase phosphorylation site. In vivo data suggest that HSFA4A can be the substrate of other kinases as well. Changing Ser309 to Asp or Ala alters intramolecular multimerization. Chromatin immunoprecipitation assays confirmed binding of HSFA4A to promoters of target genes encoding the small heat shock protein HSP17.6A and transcription factors WRKY30 and ZAT12. HSFA4A overexpression enhanced tolerance to individually and simultaneously applied heat and salt stresses through reduction of oxidative damage. Our results suggest that this heat shock factor is a component of a complex stress regulatory pathway, connecting upstream signals mediated by MAP kinases MPK3/6 and MPK4 with transcription regulation of a set of stress-induced target genes 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Arabidopsis 
650 4 |a MAP kinases 
650 4 |a combined stress 
650 4 |a heat 
650 4 |a heat shock factor A4A 
650 4 |a phosphorylation 
650 4 |a promoter binding 
650 4 |a salinity 
650 4 |a transcription regulation 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a HSFA4A protein, Arabidopsis  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a Sodium Chloride  |2 NLM 
650 7 |a 451W47IQ8X  |2 NLM 
650 7 |a AtMPK4 protein, Arabidopsis  |2 NLM 
650 7 |a EC 2.7.11.24  |2 NLM 
650 7 |a Mitogen-Activated Protein Kinases  |2 NLM 
650 7 |a EC 2.7.11.24  |2 NLM 
700 1 |a Rigó, Gábor  |e verfasserin  |4 aut 
700 1 |a Zsigmond, Laura  |e verfasserin  |4 aut 
700 1 |a Pérez-Salamó, Imma  |e verfasserin  |4 aut 
700 1 |a Papdi, Csaba  |e verfasserin  |4 aut 
700 1 |a Klement, Eva  |e verfasserin  |4 aut 
700 1 |a Pettkó-Szandtner, Aladár  |e verfasserin  |4 aut 
700 1 |a Baba, Abu Imran  |e verfasserin  |4 aut 
700 1 |a Ayaydin, Ferhan  |e verfasserin  |4 aut 
700 1 |a Dasari, Ramakrishna  |e verfasserin  |4 aut 
700 1 |a Cséplő, Ágnes  |e verfasserin  |4 aut 
700 1 |a Szabados, László  |e verfasserin  |4 aut 
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