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01000naa a22002652 4500 |
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NLM297012304 |
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DE-627 |
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20231225091058.0 |
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cr uuu---uuuuu |
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231225s2019 xx |||||o 00| ||eng c |
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7 |
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|a 10.1094/PDIS-09-18-1549-RE
|2 doi
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| 028 |
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|a pubmed24n0990.xml
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|a DE-627
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Galarneau, Erin R A
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Drought Exacerbates Botryosphaeria Dieback Symptoms in Grapevines and Confounds Host-based Molecular Markers of Infection by Neofusicoccum parvum
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|c 2019
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| 336 |
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 05.08.2019
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|a Date Revised 05.08.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Neofusicoccum parvum, causal fungus of the grapevine trunk disease Botryosphaeria dieback, attacks the wood of Vitis vinifera. Because lesions are internal, using putative host-based markers of infection from leaves for diagnosis is a nondestructive option. However, their specificity under drought stress is unknown. Potted 'Cabernet-Sauvignon' were inoculated with N. parvum in the greenhouse after wounding (IW), and with wounded and nonwounded noninoculated controls. At 2 weeks postinoculation (WPI), half of the plants were severely stressed (SS), receiving 30% water volume of the well-watered (WW) plants. Larger lesions at 12 WPI among IW-SS plants, compared with all other treatments, revealed an interactive effect of inoculation and drought on lesion length. Expression of eight putative marker genes was analyzed in leaves by qPCR at the onset of drought stress, and at 8 and 12 WPI. One marker showed consistent over-expression at 8 WPI in IW plants, regardless of water treatment, suggesting specificity to infection. By 12 WPI, higher expression of seven genes in all SS plants (across inoculation treatments) revealed specificity to drought. Cross-reactivity of markers to drought, therefore, limits their utility for disease diagnosis in the field, where drought induced by climate and deficit irrigation is common
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a climate/weather effects
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| 650 |
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|a epidemiology
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| 650 |
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4 |
|a fruit
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| 650 |
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4 |
|a fungi
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| 650 |
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4 |
|a pathogen detection
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| 650 |
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4 |
|a small fruits
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| 650 |
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7 |
|a Genetic Markers
|2 NLM
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| 700 |
1 |
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|a Lawrence, Daniel P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Travadon, Renaud
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Baumgartner, Kendra
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 103(2019), 7 vom: 09. Juli, Seite 1738-1745
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
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|g volume:103
|g year:2019
|g number:7
|g day:09
|g month:07
|g pages:1738-1745
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| 856 |
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|u http://dx.doi.org/10.1094/PDIS-09-18-1549-RE
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