Biological and Molecular Properties of Wild potato mosaic virus Isolates from Pepino (Solanum muricatum)

In 1976, a virus with flexuous, filamentous virions typical of the family Potyviridae was isolated from symptomatic pepino (Solanum muricatum) plants growing in two valleys in Peru's coastal desert region. In 2014, a virus with similar-shaped virions was isolated from asymptomatic fruits obtain...

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Veröffentlicht in:Plant disease. - 1997. - 103(2019), 7 vom: 09. Juli, Seite 1746-1756
1. Verfasser: Fribourg, Cesar E (VerfasserIn)
Weitere Verfasser: Gibbs, Adrian J, Adams, Ian P, Boonham, Neil, Jones, Roger A C
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article American lineage Peru bush fruit disease management economic significance etiology high-throughput sequencing host sensitivity to infection isolate virulence mehr... pathogen detection pathogen diversity pepino phylogenetic analysis symptomatology virions viruses and viroids widespread infection
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520 |a In 1976, a virus with flexuous, filamentous virions typical of the family Potyviridae was isolated from symptomatic pepino (Solanum muricatum) plants growing in two valleys in Peru's coastal desert region. In 2014, a virus with similar-shaped virions was isolated from asymptomatic fruits obtained from pepino plants growing in six coastal valleys and a valley in Peru's Andean highlands. Both were identified subsequently as Wild potato mosaic virus (WPMV) by serology or high-throughput sequencing (HTS). The symptoms caused by two old and seven new isolates from pepino were examined in indicator plants. Infected solanaceous hosts varied considerably in their sensitivities to infection and individual isolates varied greatly in virulence. All seven new isolates caused quick death of infected Nicotiana benthamiana plants and more than half of them killed infected plants of Physalis floridana and S. chancayense. These three species were the most sensitive to infection. The most virulent isolate was found to be BA because it killed five of eight solanaceous host species whereas CA was the least severe because it only killed N. benthamiana. Using HTS, complete genomic sequences of six isolates were obtained, with one isolate (FE) showing evidence of recombination. The distances between individual WPMV isolates in phylogenetic trees and the geographical distances between their collection sites were found to be unrelated. The individual WPMV isolates displayed nucleotide sequence identities of 80.9-99.8%, whereas the most closely related virus, Potato virus V (PVV), was around 75% identical to WPMV. WPMV, PVV, and Peru tomato virus formed clusters of similar phylogenetic diversity, and were found to be distinct but related viruses within the overall Potato virus Y lineage. WPMV infection seems widespread and of likely economic significance to pepino producers in Peru's coastal valleys. Because it constitutes the fifth virus found infecting pepino and this crop is entirely vegetatively propagated, development of healthy pepino stock programs is advocated 
650 4 |a Journal Article 
650 4 |a American lineage 
650 4 |a Peru 
650 4 |a bush fruit 
650 4 |a disease management 
650 4 |a economic significance 
650 4 |a etiology 
650 4 |a high-throughput sequencing 
650 4 |a host sensitivity to infection 
650 4 |a isolate virulence 
650 4 |a pathogen detection 
650 4 |a pathogen diversity 
650 4 |a pepino 
650 4 |a phylogenetic analysis 
650 4 |a symptomatology 
650 4 |a virions 
650 4 |a viruses and viroids 
650 4 |a widespread infection 
700 1 |a Gibbs, Adrian J  |e verfasserin  |4 aut 
700 1 |a Adams, Ian P  |e verfasserin  |4 aut 
700 1 |a Boonham, Neil  |e verfasserin  |4 aut 
700 1 |a Jones, Roger A C  |e verfasserin  |4 aut 
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773 1 8 |g volume:103  |g year:2019  |g number:7  |g day:09  |g month:07  |g pages:1746-1756 
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