|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM296778303 |
| 003 |
DE-627 |
| 005 |
20231225090556.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2019 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/nph.15882
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0989.xml
|
| 035 |
|
|
|a (DE-627)NLM296778303
|
| 035 |
|
|
|a (NLM)31058325
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Wang, Pengfei
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Electron tomography of plant organelles and the outlook for correlative microscopic approaches
|
| 264 |
|
1 |
|c 2019
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 27.02.2020
|
| 500 |
|
|
|a Date Revised 30.09.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
|
| 520 |
|
|
|a Structural analyses of organelles and localization of proteins in their confines are essential to investigate inner workings of eukaryotic cells. Electron tomography (ET) is a three-dimensional electron microscopy method with which we can extract sliced views of organelles from any direction and quantify their structural parameters at nanometer-level resolution. This advanced electron microscopy tool is suited for characterization of convoluted membrane compartments and of cellular constituents of dimensions smaller than 100 nm. ET studies of plant cells fixed by rapid freezing have expanded our understanding of the biogenesis and functions of plant organelles. Here we describe how the molecular imaging capacity of correlative light and electron microscopy can be integrated with ET in studies of plant organelles
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Review
|
| 650 |
|
4 |
|a chromatin
|
| 650 |
|
4 |
|a correlative light and electron microscopy
|
| 650 |
|
4 |
|a electron microscopy (EM)
|
| 650 |
|
4 |
|a electron tomography (ET)
|
| 650 |
|
4 |
|a endomembrane compartments
|
| 650 |
|
4 |
|a fluorescence microscopy
|
| 650 |
|
4 |
|a high-pressure freezing
|
| 650 |
|
4 |
|a plant organelle structures
|
| 700 |
1 |
|
|a Liang, Zizhen
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kang, Byung-Ho
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 223(2019), 4 vom: 20. Sept., Seite 1756-1761
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
|
| 773 |
1 |
8 |
|g volume:223
|g year:2019
|g number:4
|g day:20
|g month:09
|g pages:1756-1761
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/nph.15882
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 223
|j 2019
|e 4
|b 20
|c 09
|h 1756-1761
|