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231225s2019 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2018-0150
|2 doi
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Hansen, Mads Frederik
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Fluidic resistance control enables high-throughput establishment of mixed-species biofilms
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a Date Completed 26.02.2020
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|a Date Revised 26.02.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a Bacteria often live in communities of mixed species embedded in a self-produced extracellular matrix of polysaccharides, proteins and DNA, termed biofilms. The BioFlux microfluidic flow system is useful for studying biofilm formation in different media under flow. However, analyzing the architecture and maturation of biofilms under flow requires a proper seeding, which can prove difficult when working with bacteria of different sizes, motile bacteria or aiming for a high number of replicates. Here we developed an efficient protocol that exploits viscosity tuning and seeding indicator dyes to improve seeding and allow for high-throughput examination and visualization of consistent mono- and mixed-species biofilm developments under flow
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a BioFlux
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|a CLSM
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| 650 |
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|a biofilm analysis
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| 650 |
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|a biofilm device
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| 650 |
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4 |
|a biofilm formation
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| 650 |
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4 |
|a biofilm seeding
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| 650 |
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4 |
|a microfluidic device
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| 650 |
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4 |
|a microfluidic flow systems
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| 650 |
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4 |
|a mixed species biofilm
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| 650 |
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4 |
|a shear stress
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| 700 |
1 |
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|a Torp, Anders Meyer
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Madsen, Jonas Stenløkke
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Røder, Henriette Lyng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Burmølle, Mette
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t BioTechniques
|d 1988
|g 66(2019), 5 vom: 16. Mai, Seite 235-239
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:66
|g year:2019
|g number:5
|g day:16
|g month:05
|g pages:235-239
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|u http://dx.doi.org/10.2144/btn-2018-0150
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