A mismatch-tolerant RT-quantitative PCR : application to broad-spectrum detection of respiratory syncytial virus
Quantitative PCR (qPCR) is widely used to detect viruses. However, mismatches occurring in the 3'-end of the primers reduce amplification efficiency of qPCR and limit its capacity in detection of highly variable viruses. Here, we reported a mismatch-tolerant RT-qPCR with a small amount of addit...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 66(2019), 5 vom: 16. Mai, Seite 225-230
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| 1. Verfasser: |
Li, Yingxue
(VerfasserIn) |
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| Weitere Verfasser: |
Wan, Zhenzhou,
Hu, Yihong,
Zhou, Yi,
Chen, Qin,
Zhang, Chiyu |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2019
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
detection
high-fidelity DNA polymerase
mismatch-tolerant qPCR
respiratory syncytial virus
virus variant
DNA Primers
RNA, Viral |
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