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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2019.04.029
|2 doi
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|a pubmed25n0988.xml
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|a (DE-627)NLM296677256
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|a (NLM)31048123
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|a (PII)S0981-9428(19)30163-9
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Jakubowicz, Małgorzata
|e verfasserin
|4 aut
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| 245 |
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|a Expression profiling of CTR1-like and EIN2-like genes in buds and leaves of Populus tremula, and in vitro study of the interaction between their polypeptides
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| 264 |
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|c 2019
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 17.06.2019
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2019 Elsevier Masson SAS. All rights reserved.
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|a In Arabidopsis, the serine/threonine protein kinase Constitutive Triple Response 1 (CTR1) and Ethylene Insensitive 2 polypeptide (EIN2) functions are key negative and positive components, respectively, in the ethylene signalling route. Here, we report on an in silico study of members of the CTR1-like and EIN2-like polypeptide families from poplars. The expression of CTR1-like and EIN2-like genes such as Ptre-CTR1, Ptre-CTR3 and Ptre-EIN2a was studied in Populus tremula buds and leaves in response to dehydration, various light conditions and under senescence. In buds under dehydration, the maximal fold-change of the Ptre-CTR1, Ptre-CTR3 and Ptre-EIN2a expression level recorded almost identical values. This suggests that maintenance of a constant ratio between the transcript levels of genes encoding positive and negative ethylene signalling components is required under stress. The expression of the studied genes was 1.4-to 3-fold higher in response to darkness, but 4.5- to 51.2-fold and 21.6- to 51.2-fold higher under the early and moderate leaf senescence, respectively. It is worth noting that the senescence-related Ptre-EIN2a and Ptre-CTR3a expression profiles were very similar. Using in vitro assays, we demonstrated the ability of the catalytic domain of Ptre-CTR1 to phosphorylate the Ptre-EIN2a-like polypeptide, which is similar to that in Arabidopsis. The target substrate, the Ptre-CEND2a polypeptide (C-terminal part of Ptre-EIN2a), was only phosphorylated by the protein kinase Ptre-CTR1 and not by Ptre-CTR3. Moreover, the addition of Ptre-CTR3 polypeptides (-CTR3a or -CTR3b forms) to the reaction mixture had an inhibitory effect on Ptre-CTR1 auto- and trans-phosphorylation. In contrast to Ptre-CTR1, Ptre-CTR3 may act as a positive regulator in ethylene signalling in poplar; however, this hypothesis requires in vivo confirmation. Thus, the ethylene signalling route in poplar seems to be under the control of certain additional mechanisms which have not been reported in Arabidopsis
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|a Journal Article
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| 650 |
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4 |
|a 1-Aminocyclopropane-1-Carboxylate oxidase
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| 650 |
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|a Constitutive triple response 1
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| 650 |
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4 |
|a Ethylene
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| 650 |
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|a Ethylene insensitive 2
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| 650 |
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4 |
|a Poplar
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a EIN2 protein, Arabidopsis
|2 NLM
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| 650 |
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7 |
|a Ethylenes
|2 NLM
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| 650 |
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|a Receptors, Cell Surface
|2 NLM
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| 650 |
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|a ethylene
|2 NLM
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| 650 |
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7 |
|a 91GW059KN7
|2 NLM
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| 650 |
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|a Protein Kinases
|2 NLM
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| 650 |
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7 |
|a EC 2.7.-
|2 NLM
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| 650 |
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7 |
|a CTR1 protein, Arabidopsis
|2 NLM
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| 650 |
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7 |
|a EC 2.7.1.-
|2 NLM
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| 700 |
1 |
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|a Nowak, Witold
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gałgański, Łukasz
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Babula-Skowrońska, Danuta
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 139(2019) vom: 15. Juni, Seite 660-671
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnas
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| 773 |
1 |
8 |
|g volume:139
|g year:2019
|g day:15
|g month:06
|g pages:660-671
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| 856 |
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|u http://dx.doi.org/10.1016/j.plaphy.2019.04.029
|3 Volltext
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|a AR
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|d 139
|j 2019
|b 15
|c 06
|h 660-671
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