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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-10-18-1751-RE
|2 doi
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|a pubmed24n0987.xml
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|a eng
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| 100 |
1 |
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|a Batuman, Ozgur
|e verfasserin
|4 aut
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|a Rasta Disease of Tomato in Ghana is Caused by the Pospiviroids Potato spindle tuber viroid and Tomato apical stunt viroid
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|c 2019
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 05.08.2019
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Rasta is a virus-like disease of unknown etiology affecting tomato (Solanum lycopersicum) plants in Ghana. Symptoms include stunting; epinasty, crumpling, and chlorosis of leaves; and necrosis of leaf veins, petioles, and stems. Leaf samples with rasta symptoms were collected from commercial tomato fields in Ghana in October 2012 and applied to FTA cards, and RNA extracts were prepared. Reverse-transcription polymerase chain reaction (RT-PCR) tests with primers for Columnea latent viroid, which causes rasta-like symptoms in tomato plants in Mali, were negative, whereas tests with degenerate viroid primer pairs were inconclusive. However, tomato seedlings (Early Pak 7) mechanically inoculated with RNA extracts of 10 of 13 samples developed rasta-like symptoms. In RT-PCR tests with RNA from leaves of the 10 symptomatic seedlings and primers for Potato spindle tuber viroid (PSTVd) or Tomato apical stunt viroid (TASVd), the expected size (approximately 360 bp) of DNA fragment was amplified from eight and two seedlings, respectively. Sequence analyses confirmed that these fragments were from PSTVd and TASVd isolates, and revealed a single PSTVd haplotype and two TASVd haplotypes. The PSTVd and TASVd isolates from Ghana had high nucleotide identities (>94%) with isolates from other geographic regions. In a host range study, PSTVd and TASVd isolates from Ghana induced rasta symptoms in the highly susceptible tomato cultivar Early Pak 7 and mild or no symptoms in Glamour, and symptomless infections in a number of other solanaceous species. PSTVd and TASVd isolates were seed associated and possibly seed transmitted
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|a Journal Article
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1 |
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|a Çiftçi, Ö Cem
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Osei, Michael K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Miller, Sally A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rojas, Maria R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gilbertson, Robert L
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 103(2019), 7 vom: 11. Juli, Seite 1525-1535
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|x 0191-2917
|7 nnns
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|g volume:103
|g year:2019
|g number:7
|g day:11
|g month:07
|g pages:1525-1535
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| 856 |
4 |
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|u http://dx.doi.org/10.1094/PDIS-10-18-1751-RE
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