A quantitative method for the high throughput screening for the soil adhesion properties of plant and microbial polysaccharides and exudates

BACKGROUND AND AIMS: Understanding the structures and functions of carbon-based molecules in soils is an important goal in the context of soils as an ecosystem function of immense importance. Polysaccharides are implicated in maintaining soil aggregate status but have not been extensively dissected...

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Veröffentlicht in:Plant and soil. - 1998. - 428(2018), 1 vom: 15., Seite 57-65
1. Verfasser: Akhtar, Jumana (VerfasserIn)
Weitere Verfasser: Galloway, Andrew F, Nikolopoulos, Georgios, Field, Katie J, Knox, Paul
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Plant and soil
Schlagworte:Journal Article Bioadhesives Polysaccharides Rhizosheaths Soil Soil aggregate status Soil-adhesion Xyloglucan
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520 |a BACKGROUND AND AIMS: Understanding the structures and functions of carbon-based molecules in soils is an important goal in the context of soils as an ecosystem function of immense importance. Polysaccharides are implicated in maintaining soil aggregate status but have not been extensively dissected in terms of their structures and soil adhesion properties. This is largely because of the technical difficulties in identifying polysaccharide structures and quantifying any functional properties 
520 |a METHODS: Here, we describe the use of a novel nitrocellulose-based adhesion assay to determine the relative capacities for soil adhesion of over twenty plant and microbial polysaccharides that are likely to be present in soil and to contribute to organic matter content and properties. Weights of soil adhered to spots of known amounts of specific polysaccharides were quantified by scanning of the nitrocellulose sheets 
520 |a RESULTS: The most effective polysaccharides identified from this survey included chitosan, β-1,3-glucan, gum tragacanth, xanthan and xyloglucan. We also demonstrate that the soil adhesion assay is suitable to assess the soil-binding properties of plant exudates 
520 |a CONCLUSIONS: The soil adhesion assay will be useful for the functional dissection of the organic matter components of soils and also of the factors involved in soil attachment to plant roots and in rhizosheath formation 
650 4 |a Journal Article 
650 4 |a Bioadhesives 
650 4 |a Polysaccharides 
650 4 |a Rhizosheaths 
650 4 |a Soil 
650 4 |a Soil aggregate status 
650 4 |a Soil-adhesion 
650 4 |a Xyloglucan 
700 1 |a Galloway, Andrew F  |e verfasserin  |4 aut 
700 1 |a Nikolopoulos, Georgios  |e verfasserin  |4 aut 
700 1 |a Field, Katie J  |e verfasserin  |4 aut 
700 1 |a Knox, Paul  |e verfasserin  |4 aut 
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856 4 0 |u http://dx.doi.org/10.1007/s11104-018-3670-1  |3 Volltext 
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