Phylogeny, Distribution, and Pathogenicity of Lasiodiplodia Species Associated With Cankers and Dieback Symptoms of Persian Lime in Mexico

Persian lime (Citrus latifolia Tan.) is an important and widely cultivated fruit crop in several regions of Mexico. In recent years, severe symptoms of gummosis, stem cankers, and dieback were detected in the Persian lime-producing region in the states of Veracruz and Puebla, Mexico. The aims of thi...

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Veröffentlicht in:Plant disease. - 1997. - 103(2019), 6 vom: 01. Juni, Seite 1156-1165
1. Verfasser: Bautista-Cruz, M A (VerfasserIn)
Weitere Verfasser: Almaguer-Vargas, G, Leyva-Mir, S G, Colinas-León, M T, Correia, K C, Camacho-Tapia, M, Robles-Yerena, L, Michereff, S J, Tovar-Pedraza, J M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article Botryosphaeriaceae fungal diversity pathogenicity phylogenetic analysis DNA, Fungal
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520 |a Persian lime (Citrus latifolia Tan.) is an important and widely cultivated fruit crop in several regions of Mexico. In recent years, severe symptoms of gummosis, stem cankers, and dieback were detected in the Persian lime-producing region in the states of Veracruz and Puebla, Mexico. The aims of this study were to identify the species of Lasiodiplodia associated with these symptoms, determine the distribution of these species, and test their pathogenicity and virulence on Persian lime plants. In 2015, symptomatic samples were collected from 12 commercial Persian lime orchards, and 60 Lasiodiplodia isolates were obtained. Fungal identification of 32 representative isolates was performed using a phylogenetic analysis based on DNA sequence data of the internal transcribed spacer region and part of the translation elongation factor 1-α and β-tubulin genes. Sequence analyses were carried out using the Maximum Likelihood and Bayesian Inference methods. Six Lasiodiplodia species were identified as Lasiodiplodia pseudotheobromae, Lasiodiplodia theobromae, Lasiodiplodia brasiliense, Lasiodiplodia subglobosa, Lasiodiplodia citricola, and Lasiodiplodia iraniensis. All Lasiodiplodia species of this study are reported for the first time in association with Persian lime in Mexico and worldwide. L. pseudotheobromae (46.9% of isolates) was the most frequently isolated species followed by L. theobromae (28.1%) and L. brasiliense (12.5%). Pathogenicity on Persian lime young plants using a mycelial plug inoculation method showed that all identified Lasiodiplodia species were able to cause necrotic lesions and gummosis, but L. subglobosa, L. iraniensis, and L. pseudotheobromae were the most virulent 
650 4 |a Journal Article 
650 4 |a Botryosphaeriaceae 
650 4 |a fungal diversity 
650 4 |a pathogenicity 
650 4 |a phylogenetic analysis 
650 7 |a DNA, Fungal  |2 NLM 
700 1 |a Almaguer-Vargas, G  |e verfasserin  |4 aut 
700 1 |a Leyva-Mir, S G  |e verfasserin  |4 aut 
700 1 |a Colinas-León, M T  |e verfasserin  |4 aut 
700 1 |a Correia, K C  |e verfasserin  |4 aut 
700 1 |a Camacho-Tapia, M  |e verfasserin  |4 aut 
700 1 |a Robles-Yerena, L  |e verfasserin  |4 aut 
700 1 |a Michereff, S J  |e verfasserin  |4 aut 
700 1 |a Tovar-Pedraza, J M  |e verfasserin  |4 aut 
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