A robust fractionation method for protein subcellular localization studies in Escherichia coli
Fractionation in Gram-negative bacteria is used to identify the subcellular localization of proteins, in particular the localization of exported recombinant proteins. The process of cell fractionation can be fraught with cross-contamination issues and often lacks supporting data for fraction purity....
| Veröffentlicht in: | BioTechniques. - 1993. - 66(2019), 4 vom: 01. Apr., Seite 171-178 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2019
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't alkaline phosphatase cold-osmotic shock export fractionation periplasm Escherichia coli Proteins Recombinant Proteins |
| Online verfügbar |
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