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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b03744
|2 doi
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|a pubmed24n0985.xml
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|a (NLM)30955336
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Bai, Lihui
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Saccharides Create a Crowding Environment for Gene Expression in Cell-Free Systems
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 27.07.2020
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|a Date Revised 27.07.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Cellular physical microenvironment such as crowding shows great influence on enzymatic reactions. Herein, we report a new finding that saccharides with low molecular weight create an effective crowding microenvironment for gene expression in cell-free protein synthesis, which provides valuable implications for living systems. Four saccharides including sorbose, galactose, sucrose, and cellobiose are screened out as effective crowders. At a low concentration range of saccharides, both the mRNA and protein amounts present an upward trend with the concentration increment of saccharides; when the concentrations exceed a critical value, the mRNA and protein amounts decrease. A mechanism is proposed that at low concentrations of saccharides, the effective concentrations of reactants increase due to the coexistence of crowders and reactants in a finite volume; when the concentrations exceed a critical value, the molecular diffusion of reactants is dominantly restricted due to the increased viscosity. Our finding opens a new view that saccharides with low molecular weight could be crowders and provides a new insight that substances with low molecular weight in cells would produce a crowding effect on biochemical reactions in living systems
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a RNA, Messenger
|2 NLM
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|a Sugars
|2 NLM
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|a enhanced green fluorescent protein
|2 NLM
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|a Green Fluorescent Proteins
|2 NLM
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|a 147336-22-9
|2 NLM
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1 |
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|a Guo, Xiaocui
|e verfasserin
|4 aut
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|a Zhang, Xue
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Wenting
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Dayong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 35(2019), 17 vom: 30. Apr., Seite 5931-5936
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:35
|g year:2019
|g number:17
|g day:30
|g month:04
|g pages:5931-5936
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|u http://dx.doi.org/10.1021/acs.langmuir.8b03744
|3 Volltext
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