Key changes in gene expression identified for different stages of C4 evolution in Alloteropsis semialata

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 70(2019), 12 vom: 28. Juni, Seite 3255-3268
1. Verfasser:	Dunning, Luke T (VerfasserIn)
Weitere Verfasser:	Moreno-Villena, Jose J, Lundgren, Marjorie R, Dionora, Jacqueline, Salazar, Paolo, Adams, Claire, Nyirenda, Florence, Olofsson, Jill K, Mapaura, Anthony, Grundy, Isla M, Kayombo, Canisius J, Dunning, Lucy A, Kentatchime, Fabrice, Ariyarathne, Menaka, Yakandawala, Deepthi, Besnard, Guillaume, Quick, W Paul, Bräutigam, Andrea, Osborne, Colin P, Christin, Pascal-Antoine
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2019
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Adaptation C4 photosynthesis complex trait intermediates phylogenetics transcriptomics Carbon 7440-44-0

Beschreibung
Zusammenfassung:	© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. C4 photosynthesis is a complex trait that boosts productivity in tropical conditions. Compared with C3 species, the C4 state seems to require numerous novelties, but species comparisons can be confounded by long divergence times. Here, we exploit the photosynthetic diversity that exists within a single species, the grass Alloteropsis semialata, to detect changes in gene expression associated with different photosynthetic phenotypes. Phylogenetically informed comparative transcriptomics show that intermediates with a weak C4 cycle are separated from the C3 phenotype by increases in the expression of 58 genes (0.22% of genes expressed in the leaves), including those encoding just three core C4 enzymes: aspartate aminotransferase, phosphoenolpyruvate carboxykinase, and phosphoenolpyruvate carboxylase. The subsequent transition to full C4 physiology was accompanied by increases in another 15 genes (0.06%), including only the core C4 enzyme pyruvate orthophosphate dikinase. These changes probably created a rudimentary C4 physiology, and isolated populations subsequently improved this emerging C4 physiology, resulting in a patchwork of expression for some C4 accessory genes. Our work shows how C4 assembly in A. semialata happened in incremental steps, each requiring few alterations over the previous step. These create short bridges across adaptive landscapes that probably facilitated the recurrent origins of C4 photosynthesis through a gradual process of evolution
Beschreibung:	Date Completed 13.07.2020 Date Revised 12.10.2023 published: Print Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erz149