Induction of isoflavonoid biosynthesis in Lotus japonicus after UV-B irradiation

Copyright © 2019 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 236(2019) vom: 25. Mai, Seite 88-95
1. Verfasser: Kaducová, Mária (VerfasserIn)
Weitere Verfasser: Monje-Rueda, María Dolores, García-Calderón, Margarita, Pérez-Delgado, Carmen María, Eliášová, Adriana, Gajdošová, Silvia, Petruľová, Veronika, Betti, Marco, Márquez, Antonio J, Paľove-Balang, Peter
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Flavonoid Isoflavonoid Lotus japonicus Stress-defence UV radiation Vestitol Free Radical Scavengers Isoflavones Phenylalanine Ammonia-Lyase EC 4.3.1.24
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520 |a Enhanced ultraviolet radiation (UV) is an important environmental factor that may cause reductions in the growth and productivity of plants. In the present work we studied the response to UV-B radiation in leaves of the model legume Lotus japonicus. After UV-B treatment, induction of phenyalanine-ammonia lyase gene expression and enzyme activity was detected. Among the ten genes encoding for PAL found in the L. japonicus genome, LjPAL1 was both the most expressed and the most induced. All the genes encoding for enzymes of the isoflavonoid pathway were also strongly induced; this was paralleled by a marked accumulation of vestitol and isoliquiritigenin. Moreover, accumulation of several other isoflavonoids was also detected. In vitro measurements of the free radical scavenging capacity of vestitol indicated that this compound can be an appropriate free radical scavenger, suggesting a possible role for this molecule in the response to abiotic stress. On the other hand, an increase of flavonol levels was not observed while the expression of the key enzymes for flavonol biosynthesis flavanone-3-hydroxylase and flavonol synthase was decreased. Taken together, these results indicate that L. japonicus follows a peculiar strategy in its response to UV radiation by accumulating isoflavonoids as an possible alternative to accumulation of flavonols as observed in other plant species 
650 4 |a Journal Article 
650 4 |a Flavonoid 
650 4 |a Isoflavonoid 
650 4 |a Lotus japonicus 
650 4 |a Stress-defence 
650 4 |a UV radiation 
650 4 |a Vestitol 
650 7 |a Free Radical Scavengers  |2 NLM 
650 7 |a Isoflavones  |2 NLM 
650 7 |a Phenylalanine Ammonia-Lyase  |2 NLM 
650 7 |a EC 4.3.1.24  |2 NLM 
700 1 |a Monje-Rueda, María Dolores  |e verfasserin  |4 aut 
700 1 |a García-Calderón, Margarita  |e verfasserin  |4 aut 
700 1 |a Pérez-Delgado, Carmen María  |e verfasserin  |4 aut 
700 1 |a Eliášová, Adriana  |e verfasserin  |4 aut 
700 1 |a Gajdošová, Silvia  |e verfasserin  |4 aut 
700 1 |a Petruľová, Veronika  |e verfasserin  |4 aut 
700 1 |a Betti, Marco  |e verfasserin  |4 aut 
700 1 |a Márquez, Antonio J  |e verfasserin  |4 aut 
700 1 |a Paľove-Balang, Peter  |e verfasserin  |4 aut 
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773 1 8 |g volume:236  |g year:2019  |g day:25  |g month:05  |g pages:88-95 
856 4 0 |u http://dx.doi.org/10.1016/j.jplph.2019.03.003  |3 Volltext 
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