Rapid identification of genome-edited mesenchymal stem cell colonies via Cas9

Mesenchymal stem cells (MSCs) have been intensively investigated and widely applied in regenerative medicine and immune modulation. However, their efficacy declines during the aging or disease process. Thus, genome-edited MSCs with over-expression or inhibition of specific genes hold a great deal of...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 66(2019), 5 vom: 09. Mai, Seite 231-234
1. Verfasser: Jiang, Wei (VerfasserIn)
Weitere Verfasser: Lian, Wei, Chen, Jieting, Li, Wenlei, Huang, Jieyong, Lai, Baoyu, Li, Lingyun, Huang, Zhong, Xu, Jianyong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't AAVS1 Cas9 IL-10 IL-37 MSC MSC colony direct PCR genome editing mehr... mesenchymal stem cells rapid identification Interleukin-10 130068-27-8 CRISPR-Associated Protein 9 EC 3.1.-