Rice FLOURY ENDOSPERM10 encodes a pentatricopeptide repeat protein that is essential for the trans-splicing of mitochondrial nad1 intron 1 and endosperm development

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 223(2019), 2 vom: 05. Juli, Seite 736-750
1. Verfasser:	Wu, Mingming (VerfasserIn)
Weitere Verfasser:	Ren, Yulong, Cai, Maohong, Wang, Yunlong, Zhu, Shanshan, Zhu, Jianping, Hao, Yuanyuan, Teng, Xuan, Zhu, Xiaopin, Jing, Ruonan, Zhang, Huan, Zhong, Mingsheng, Wang, Yongfei, Lei, Cailin, Zhang, Xin, Guo, Xiuping, Cheng, Zhijun, Lin, Qibing, Wang, Jie, Jiang, Ling, Bao, Yiqun, Wang, Yihua, Wan, Jianmin
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2019
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't nad1 FLO10 aleurone layer endosperm development intron splicing mitochondria pentatricopeptide repeat (PPR) rice mehr... Plant Proteins RNA, Messenger aleurone Starch 9005-25-8 Electron Transport Complex I EC 7.1.1.2

Beschreibung
Zusammenfassung:	© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust. Endosperm, the major storage organ in cereal grains, determines grain yield and quality. Despite the fact that a role for P-type pentatricopeptide repeat (PPR) proteins in the regulation of endosperm development has emerged, molecular functions of many P-type PPR proteins remain obscure. Here, we report a rice endosperm defective mutant, floury endosperm10 (flo10), which developed smaller starch grains in starchy endosperm and abnormal cells in the aleurone layer. Map-based cloning and rescued experiments showed that FLO10 encodes a P-type PPR protein with 26 PPR motifs, which is localized to mitochondria. Loss of function of FLO10 affected the trans-splicing of the mitochondrial nad1 intron 1, which was accompanied by the increased accumulation of the nad1 exon 1 and exons 2-5 precursors. The failed formation of mature nad1 led to a dramatically decreased assembly and activity of complex I, reduced ATP production, and changed mitochondrial morphology. In addition, loss of function of FLO10 significantly induced an alternative respiratory pathway involving alternative oxidase. These results reveal that FLO10 plays an important role in the maintenance of mitochondrial function and endosperm development through its effect on the trans-splicing of the mitochondrial nad1 intron 1 in rice
Beschreibung:	Date Completed 02.03.2020 Date Revised 30.09.2020 published: Print-Electronic GENBANK: FLO10, AT5G15280 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.15814