|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM294940189 |
| 003 |
DE-627 |
| 005 |
20231225082555.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s1997 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1094/PDIS.1997.81.1.112A
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0983.xml
|
| 035 |
|
|
|a (DE-627)NLM294940189
|
| 035 |
|
|
|a (NLM)30870930
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Msikita, W
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a First Report of Curvularia lunata Associated with Stem Disease of Cassava
|
| 264 |
|
1 |
|c 1997
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Revised 20.11.2019
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status PubMed-not-MEDLINE
|
| 520 |
|
|
|a During surveys covering 60 cassava (Manihot esculenta Crantz) fields, randomly selected (between latitude 4°55'N and 8°16'N) in south Ghana, and 27 fields in southeast (between 4°50'N and 7°56'N) Nigeria, 8-month-old or older stems of some cassava genotypes were found to be covered by grayish brown lesions, predominantly on lignified portions of stems. Field disease incidence ranged from 0 to 80%, and severity from no disease to highly affected (>15 lesions per stem). To identify the pathogen, infected stem portions were cut out, surface disinfected, and cultured on potato dextrose agar (PDA) acidified with 0.4% (vol/vol) lactic acid. After 1 week, mycelia, conidiophores, and conidia were observed under a microscope, and the pathogen was identified as Curvularia lunata (Wakk.) Boedijn (confirmed also by the International My-cological Institute, Surrey, U.K.). To complete Koch's postulates, stem pieces of four cassava cultivars (Agric, Tchukunochi, TMS 30572, and Ben 86052), were disinfected in hot water (52°C for 5 min), transplanted in sterilized sand, and maintained in a greenhouse under natural light at 28 to 30°C. Before planting, five stems were wound inoculated (sliced with an epidermal scalpel) just above nodes, and a 5-mm-diameter PDA mycelial plug of C. lunata was applied to each wound or directly to unwounded nodes. Stems were then kept in a plastic bag for 24 h before planting. For each cultivar, five control stem pieces were similarly wounded but not treated with PDA plugs. All plants were maintained under >90% relative humidity. Control plants remained symptom-free whereas lesions and bud necrosis similar to field symptoms were observed on all inoculated plants within 1 month. Symptom development was quicker (2 to 3 weeks) on wound-inoculated than on nonwounded stems. Mortality of artificially wound-inoculated buds ranged between 30 and 100%, depending on genotype and manner of inoculation. Artificially infected stem and bud portions plated on PDA consistently yielded C. lunata. Bud sprouting of naturally infected cuttings was monitored over a period of 4 weeks after stem planting. When buds were completely colonized, sprouting was completely inhibited. However, partially colonized buds sprouted, but growth was reduced by 20 to 50% (depending on genotype), compared with healthy stems. This is the first report of C. lunata pathogenic on cassava
|
| 650 |
|
4 |
|a Journal Article
|
| 700 |
1 |
|
|a Yaninek, J S
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ahounou, M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Baimey, H
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Fagbemissi, R
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 81(1997), 1 vom: 30. Jan., Seite 112
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
|
| 773 |
1 |
8 |
|g volume:81
|g year:1997
|g number:1
|g day:30
|g month:01
|g pages:112
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1094/PDIS.1997.81.1.112A
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 81
|j 1997
|e 1
|b 30
|c 01
|h 112
|