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|a Hiseni, Pranvera
|e verfasserin
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|a Liquid array diagnostics
|b a novel method for rapid detection of microbial communities in single-tube multiplex reactions
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|c 2019
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|a published: Print
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|a Citation Status MEDLINE
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|a We present a novel liquid array diagnostics (LAD) method, which enables rapid and inexpensive detection of microbial markers in a single-tube multiplex reaction. We evaluated LAD both on pure cultures, and on infant gut microbiota for a 15-plex reaction. LAD showed more than 80% accuracy of classification and a detection limit lower than 2% of the Illumina reads per sample. The results on the clinical dataset showed that there was a rapid decrease of staphylococci from 10-day- to 4-month-old children, a peak of bifidobacteria at 4 months, and a peak of Bacteroides in 2-year-old children, which is in accordance with findings described in the literature. Being able to detect up to 50 biomarkers, LAD is a suitable method for assays where high throughput is essential
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|a Wilson, Robert C
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|a Storrø, Ola
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|i Enthalten in
|t BioTechniques
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|g 66(2019), 3 vom: 01. März, Seite 143-149
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|g volume:66
|g year:2019
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