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231225s1997 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS.1997.81.5.489
|2 doi
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|a pubmed24n0982.xml
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|a (DE-627)NLM294851992
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|a (NLM)30861927
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Loebenstein, G
|e verfasserin
|4 aut
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|a Improved Detection of Potato Leafroll Luteovirus in Leaves and Tubers with a Digoxigenin-Labeled cRNA Probe
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|c 1997
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a A digoxigenin-labeled cRNA probe of approximately 2,100 bp was more than 2,000 times more sensitive in detecting potato leafroll virus (PLRV) in leaf extracts of Datura stramonium, Physalis floridana, and potatoes than enzyme-linked immunosorbent assay (ELISA). The limit of detecting PLRV with the probe was 1 pg/ml compared with 2 ng/ml by ELISA. The probe detected PLRV easily in dormant tuber tissues at dilutions of up to 1:100. There was no background reaction with healthy extracts. No reactions were observed between the probe and potato X potexvirus or potato Y potyvirus
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|a Journal Article
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|a fluorescent-labeled probe
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|a Akad, F
|e verfasserin
|4 aut
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|a Filatov, V
|e verfasserin
|4 aut
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|a Sadvakasova, G
|e verfasserin
|4 aut
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1 |
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|a Manadilova, A
|e verfasserin
|4 aut
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1 |
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|a Bakelman, H
|e verfasserin
|4 aut
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|a Teverovsky, E
|e verfasserin
|4 aut
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|a Lachmann, O
|e verfasserin
|4 aut
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|a David, A
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant disease
|d 1997
|g 81(1997), 5 vom: 30. Mai, Seite 489-491
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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|g volume:81
|g year:1997
|g number:5
|g day:30
|g month:05
|g pages:489-491
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|u http://dx.doi.org/10.1094/PDIS.1997.81.5.489
|3 Volltext
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|d 81
|j 1997
|e 5
|b 30
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|h 489-491
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