|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM294851801 |
| 003 |
DE-627 |
| 005 |
20231225082359.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s1997 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1094/PDIS.1997.81.7.831B
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0982.xml
|
| 035 |
|
|
|a (DE-627)NLM294851801
|
| 035 |
|
|
|a (NLM)30861908
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Guzman, P
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a First Report of Bean Common Mosaic Necrosis Potyvirus (BCMNV) Infecting Common Bean in California
|
| 264 |
|
1 |
|c 1997
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Revised 20.11.2019
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status PubMed-not-MEDLINE
|
| 520 |
|
|
|a During the 1996 growing season (June to September) an outbreak of bean common mosaic was detected in a navy bean field (cv. Snow Bunting) in Colusa County, CA. Early field inspections (August 1996) revealed an incidence of 5 to 10% infection, whereas a late field inspection (September) showed an incidence of 70 to 90% infection. Enzyme-linked immunosorbent assay (ELISA) was performed on 18 leaf samples from symptomatic plants collected from this field with two monoclonal antibodies (Mab): Mab I-2, which detects bean common mosaic necrosis virus (BCMNV) strains (previously necrotic or serotype A bean common mosaic potyvirus [BCMV] strains), and Mab 197, which detects BCMV strains (previously non-necrotic or serotype B BCMV strains) and BCMNV (3). ELISA results indicated BCMNV infection in all 18 samples. In order to confirm ELISA results and to further characterize the viral isolate(s), primary leaves of the differential bean cvs. Black Turtle Soup (BTS) T-39, Topcrop, Amanda, and Sutter Pink were inoculated mechanically with sap prepared from the same leaves used for ELISA. Within 1 week, BTS T-39 and Topcrop plants showed necrotic spots on inoculated leaves and systemic necrosis and death (black root rot symptoms), Sutter Pink showed typical systemic mosaic symptoms, and Amanda showed necrotic spots and restricted vein necrosis on inoculated leaves. These reactions were consistent with infection by the NL-3 strain of BCMNV (1). Reverse transcriptase-polymerase chain reaction was used to amplify a portion of the genome of the virus that contains the 3' end of the coat protein (CP) gene and the 3' untranslated region (UTR). A DNA fragment of approximately 670 bp was amplified and DNA sequence analysis revealed that the nucleotide sequences of the 3' end of the CP and the UTR region of the California BCMNV isolate were 98 and 94% similar to those of the Michigan isolate of the BCMNV NL-3 strain (2), respectively. Together, these results suggest that the outbreak of bean common mosaic in the cv. Snow Bunting navy beans was caused by a pathogroup VI BCMNV isolate, and DNA sequence information suggests that it is similar to the NL-3 strain of BCMNV. This is the first report of BCMNV in California. References: (1) E. Drijfhout et al. Neth. J. Plant Pathol. 84:13, 1978. (2) G. F. Fang et al. Virus Res. 39:13, 1995. (3) G. I. Mink et al. Arch. Virol. S:397, 1992
|
| 650 |
|
4 |
|a Journal Article
|
| 700 |
1 |
|
|a Rojas, M R
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Davis, R M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kimble, K
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Stewart, R
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Sundstrom, F J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Gilbertson, R L
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 81(1997), 7 vom: 30. Juli, Seite 831
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
|
| 773 |
1 |
8 |
|g volume:81
|g year:1997
|g number:7
|g day:30
|g month:07
|g pages:831
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1094/PDIS.1997.81.7.831B
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 81
|j 1997
|e 7
|b 30
|c 07
|h 831
|