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231225s1997 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS.1997.81.3.254
|2 doi
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|a eng
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| 100 |
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|a Schneider, B
|e verfasserin
|4 aut
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| 245 |
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|a Detection of Phytoplasmas in Declining Pears in Southern Australia
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|c 1997
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a Forty-nine pear tree samples collected in Victoria, most of them showing decline symptoms, were tested by polymerase chain reaction (PCR) analysis to detect phytoplasmas. Two universal phytoplasma-specific primer pairs, fP1/rP7 and fU5/rU3, were tested, but only fU5/rU3 amplified the phytoplasma DNA adequately. Nested PCR with universal and group-specific primers, however, proved more effective. Thirty pear trees reacted positively in a nested PCR assay. Restriction fragment length polymorphism (RFLP) analysis with the restriction enzymes MseI and AluI of the PCR fragment amplified with the primer pair fU5/rU3 revealed patterns identical to those from the sweet potato little leaf phytoplasma. This is the first report of a phytoplasma in pear in Australia
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| 650 |
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|a Journal Article
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|a Gibb, K S
|e verfasserin
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|d 1997
|g 81(1997), 3 vom: 30. März, Seite 254-258
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|g year:1997
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|g day:30
|g month:03
|g pages:254-258
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