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231225s1998 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS.1998.82.8.871
|2 doi
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|a eng
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|a Zhang, Yun-Ping
|e verfasserin
|4 aut
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|a Analysis of Double-Stranded RNAs from Cherry Trees with Stem Pitting in California
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|c 1998
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a Five distinct dsRNA species were recovered from Bing sweet cherry (Prunus avium (L.) L.) trees with stem pitting symptoms. A 4.7-kilobase pair (kbp) dsRNA was isolated from mahaleb rootstock (P. mahaleb L.); an unrelated 4.7-kbp dsRNA, always co-purified with a 1.3-kbp dsRNA, and a 9-kbp dsRNA were from Bing cherry. In addition, an 8.5-kbp dsRNA found in diseased Shirofugen flowering cherry and in Bing cherry was identified as sour cherry green ring mottle virus (CGRMV). The larger, 8.5- and 9.0-kbp dsRNA species were graft-transmissible, while the smaller ones were non-transmissible and appeared cryptic in nature. Reverse transcription-polymerase chain reaction (RT-PCR) assays were developed for each dsRNA species by cloning and sequencing cDNA synthesized from the dsRNA templates. When several diseased collections were assayed by RT-PCR, approximately 14% reacted positively with primers for the 9.0-kbp dsRNA or CGRMV. Although CGRMV and the 9.0-kbp dsRNA caused wood-marking symptoms in graft-inoculated Mazzard (P. avium) seedling trees, no xylem or canopy symptoms developed in grafted Bing cherry. The causal agent or agents of cherry stem pitting have not been identified
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|a Journal Article
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1 |
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|a Uyemoto, J K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kirkpatrick, B C
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 82(1998), 8 vom: 28. Aug., Seite 871-874
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnas
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| 773 |
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|g volume:82
|g year:1998
|g number:8
|g day:28
|g month:08
|g pages:871-874
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|u http://dx.doi.org/10.1094/PDIS.1998.82.8.871
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