|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM29471748X |
| 003 |
DE-627 |
| 005 |
20240108141504.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2019 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/nph.15720
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1248.xml
|
| 035 |
|
|
|a (DE-627)NLM29471748X
|
| 035 |
|
|
|a (NLM)30847946
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Ishii, Takayoshi
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a RNA-guided endonuclease - in situ labelling (RGEN-ISL)
|b a fast CRISPR/Cas9-based method to label genomic sequences in various species
|
| 264 |
|
1 |
|c 2019
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 27.02.2020
|
| 500 |
|
|
|a Date Revised 04.01.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
|
| 520 |
|
|
|a Visualising the spatio-temporal organisation of the genome will improve our understanding of how chromatin structure and function are intertwined. We developed a tool to visualise defined genomic sequences in fixed nuclei and chromosomes based on a two-part guide RNA with a recombinant Cas9 endonuclease complex. This method does not require any special construct or transformation method. In contrast to classical fluorescence in situ hybridiaztion, RGEN-ISL (RNA-guided endonuclease - in situ labelling) does not require DNA denaturation, and therefore permits a better structural chromatin preservation. The application of differentially labelled trans-activating crRNAs allows the multiplexing of RGEN-ISL. Moreover, this technique is combinable with immunohistochemistry. Real-time visualisation of the CRISPR/Cas9-mediated DNA labelling process revealed the kinetics of the reaction. The broad range of adaptability of RGEN-ISL to different temperatures and combinations of methods has the potential to advance the field of chromosome biology
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a FISH
|
| 650 |
|
4 |
|a CRISPR/Cas9
|
| 650 |
|
4 |
|a centromere
|
| 650 |
|
4 |
|a immunostaining
|
| 650 |
|
4 |
|a in situ labelling
|
| 650 |
|
4 |
|a telomere
|
| 650 |
|
7 |
|a RNA, Guide, CRISPR-Cas Systems
|2 NLM
|
| 650 |
|
7 |
|a CRISPR-Associated Protein 9
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.-
|2 NLM
|
| 650 |
|
7 |
|a Endonucleases
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.-
|2 NLM
|
| 700 |
1 |
|
|a Schubert, Veit
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Khosravi, Solmaz
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Dreissig, Steven
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Metje-Sprink, Janina
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Sprink, Thorben
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Fuchs, Jörg
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Meister, Armin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Houben, Andreas
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 222(2019), 3 vom: 26. Mai, Seite 1652-1661
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
|
| 773 |
1 |
8 |
|g volume:222
|g year:2019
|g number:3
|g day:26
|g month:05
|g pages:1652-1661
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/nph.15720
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 222
|j 2019
|e 3
|b 26
|c 05
|h 1652-1661
|