RT-PCR Method for Detecting Cowpea Mottle Carmovirus in Vigna Germ Plasm

A highly sensitive reverse transcription-polymerase chain reaction (RT-PCR) method was developed to detect cowpea mottle carmovirus (CPMoV) in newly acquired germ plasm of Vigna spp. It detected virus in tissues diluted up to 10-9. The preferred primers were designed from the RNA replicase cDNA sequ...

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Bibliographische Detailangaben
Veröffentlicht in:Plant disease. - 1997. - 83(1999), 7 vom: 12. Juli, Seite 639-643
1. Verfasser: Gillaspie, A G Jr (VerfasserIn)
Weitere Verfasser: Mitchell, S E, Stuart, G W, Bozarth, R F
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article