|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM294694587 |
| 003 |
DE-627 |
| 005 |
20231225082037.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s1999 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1094/PDIS.1999.83.7.627
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0982.xml
|
| 035 |
|
|
|a (DE-627)NLM294694587
|
| 035 |
|
|
|a (NLM)30845613
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Bonde, M R
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Comparison of Effects of Acidic Electrolyzed Water and NaOCl on Tilletia indica Teliospore Germination
|
| 264 |
|
1 |
|c 1999
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Revised 20.11.2019
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status PubMed-not-MEDLINE
|
| 520 |
|
|
|a Definitive identification of free teliospores of Tilletia indica, causal agent of Karnal bunt of wheat, requires polymerase chain reaction (PCR)-based diagnostic tests. Since direct PCR amplification from teliospores has not been reliable, teliospores first must be germinated in order to obtain adequate DNA. We have routinely surface-sterilized teliospores for 2 min with 0.4% (vol/vol) sodium hypochlorite (NaOCl) to stimulate germination and produce axenic cultures. However, we observed that some spores were killed even with a 2-min NaOCl treatment, the shortest feasible duration. Decreasing the NaOCl concentration in our study from 0.4% to 0.3 and 0.2%, respectively, increased teliospore germination, but treatment times longer than 2 min still progressively reduced the germination percentages. In testing alternative methods, we found "acidic electrolyzed water" (AEW), generated by electrolysis of a weak solution of sodium chloride, also surface-sterilized and increased the rate of T. indica teliospore germination. In a representative experiment comparing the two methods, NaOCl (0.4%) for 2 min and AEW for 30 min increased germination from 19% (control) to 41 and 54%, respectively, by 7 days after treatment. Because teliospores can be treated with AEW for up to 2 h with little, if any, loss of viability, compared with 1 to 2 min for NaOCl, treatment with AEW has certain advantages over NaOCl for surface sterilizing and increasing germination of teliospores of suspect T. indica
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a disinfestant
|
| 650 |
|
4 |
|a superoxidized water
|
| 650 |
|
4 |
|a surface sterilization
|
| 700 |
1 |
|
|a Nester, S E
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Khayat, A
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Smilanick, J L
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Frederick, R D
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Schaad, N W
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 83(1999), 7 vom: 12. Juli, Seite 627-632
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
|
| 773 |
1 |
8 |
|g volume:83
|g year:1999
|g number:7
|g day:12
|g month:07
|g pages:627-632
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1094/PDIS.1999.83.7.627
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 83
|j 1999
|e 7
|b 12
|c 07
|h 627-632
|