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231225s1998 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS.1998.82.11.1276
|2 doi
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|a pubmed24n0982.xml
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|a (DE-627)NLM294692657
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|a (NLM)30845419
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Nikolaeva, Olga V
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Serological Differentiation of the Citrus Tristeza Virus Isolates Causing Stem Pitting in Sweet Orange
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| 264 |
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|c 1998
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a Citrus tristeza virus (CTV) complex comprises a number of isolates or strains producing several economically important disease syndromes in commercial Citrus spp. The stem pitting syndrome is the most important, and causes substantial losses in many citrus-producing regions of the world. In an attempt to develop a serological tool to rapidly differentiate stem pitting isolates of CTV, we evaluated many combinations of trapping and detecting antibodies in an indirect double-antibody sandwich (I-DAS) enzyme-linked immunosorbent assay (ELISA). Two combinations of trapping and detecting antibodies were found suitable for differentiating stem pitting isolates in extracts of infected sweet orange plants. One used a polyclonal serum raised against bacterially expressed CTV coat protein (CP) for trapping and a conformational monoclonal antibody 3E10 for detection, and the other used two polyclonal antisera generated against bacterially expressed CTV CP. Seventy-six CTV isolates from 20 countries, including 35 that cause stem pitting in sweet orange plants, were analyzed in I-DAS-ELISA using different combinations of polyclonal and monoclonal antibodies for trapping and as intermediate detecting antibodies. The ELISA format developed produces a strong positive signal for CTV isolates that cause stem pitting in sweet orange plants and a negative ELISA signal for CTV isolates that do not cause stem pitting. When combined with data on a universal ELISA format, i.e., reacting with a broad range of CTV isolates, these selective ELISA formats allowed reliable serological differentiation of CTV isolates that caused stem pitting in infected sweet orange plants
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| 650 |
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4 |
|a Journal Article
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| 700 |
1 |
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|a Karasev, Alexander V
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Garnsey, Stephen M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Richard F
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 82(1998), 11 vom: 12. Nov., Seite 1276-1280
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
8 |
|g volume:82
|g year:1998
|g number:11
|g day:12
|g month:11
|g pages:1276-1280
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1094/PDIS.1998.82.11.1276
|3 Volltext
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| 912 |
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 82
|j 1998
|e 11
|b 12
|c 11
|h 1276-1280
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