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|a 10.1094/PDIS.2000.84.4.492B
|2 doi
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|a eng
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|a Harmon, P
|e verfasserin
|4 aut
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|a First Report of Gray Leaf Spot on Perennial Ryegrass in Indiana
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|c 2000
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a Pyricularia grisea, the causal agent of gray leaf spot on turfgrass, was isolated from symptomatic perennial ryegrass (Lolium perenne) leaves collected from a golf course in north-central Indiana in August 1999. Gray leaf spot is an emerging threat to stands of perennial ryegrass in the mid-Atlantic and Midwestern United States (1). Symptoms were first evident in taller (6 cm) mown, rough areas surrounding golf course fairways. Field symptoms included diffuse patches (1 to 4 m in diameter) of thin, yellow-tan turf. Within larger affected areas, some of the turf was dead and matted. Close inspection revealed the presence of typical tan-gray lesions with brown margins and fish hook-like distortion of infected leaf blade tips. Incubation of affected turf in a saturated environment at 23°C for 16 h resulted in production of numerous three-celled, pear-shaped conidia characteristic of those produced by P. grisea. A pure culture of the isolate was grown on V8-juice agar in darkness at 29°C. After 10 days, the culture was exposed to continuous light for 4 days at 23°C to induce sporulation. Conidia were washed from the colony surface with sterile distilled water. Two-week-old perennial ryegrass plants in 8-cm-diameter pots were inoculated with the conidial suspension. Typical gray leaf spot symptoms resulted after incubation of inoculated plants at 27°C for 72 h in a saturated atmosphere. Uninoculated control plants exposed to the same environmental conditions remained healthy. This is the first report of gray leaf spot on perennial ryegrass in Indiana. Reference: (1) P. J. Landschoot and B. F. Hoyland. Plant Dis. 76:1280, 1992
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|a Journal Article
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|a Rane, K
|e verfasserin
|4 aut
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|a Ruhl, G
|e verfasserin
|4 aut
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|a Latin, R
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant disease
|d 1997
|g 84(2000), 4 vom: 30. Apr., Seite 492
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|x 0191-2917
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|g volume:84
|g year:2000
|g number:4
|g day:30
|g month:04
|g pages:492
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|u http://dx.doi.org/10.1094/PDIS.2000.84.4.492B
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