Evaluation of Continuous Application Technology for Delivery of the Biocontrol Agent Pseudomonas putida 06909-rif/nal

We evaluated a commercial field fermentor as a means of culturing and delivering a bacterial biocontrol agent, Pseudomonas putida 06909-rif/nal, through irrigation water. There was no evidence of contamination in 12-hour cultures produced by the fermentor. It produced 120 liters of inoculum at 5 × 1...

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Veröffentlicht in:Plant disease. - 1997. - 85(2001), 4 vom: 31. Apr., Seite 387-392
1. Verfasser: Steddom, K (VerfasserIn)
Weitere Verfasser: Menge, J A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2001
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article
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520 |a We evaluated a commercial field fermentor as a means of culturing and delivering a bacterial biocontrol agent, Pseudomonas putida 06909-rif/nal, through irrigation water. There was no evidence of contamination in 12-hour cultures produced by the fermentor. It produced 120 liters of inoculum at 5 × 108 CFU/ml after 12-hour fermentations, allowing two applications per day. Dilutions up to 1:100,000 of inoculum of P. putida 06909-rif/nal produced by the fermentor allowed the biocontrol agent to effectively colonize soil in greenhouse trials. Bacteria produced in 19-hour fermentations colonized soil better than bacteria produced in 12-hour fermentations. Ten repetitive applications of P. putida 06909-rif/nal yielded soil population levels similar to those from a single application at 10-fold greater concentrations. Repetitive applications of lower concentrations may be a cost-effective method for delivering bacterial biocontrol agents to large acreages. This work suggests that the commercial field fermentor would be a viable instrument for delivering bacterial biocontrol agents for commercial-scale field applications 
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