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|a 10.1094/PDIS.2001.85.11.1181
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|a eng
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|a Gillaspie, A G
|c Jr
|e verfasserin
|4 aut
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| 245 |
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|a RT-PCR Detection of Seedborne Cowpea aphid-borne mosaic virus in Peanut
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|c 2001
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| 336 |
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|a Text
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a The Brazilian strain of Cowpea aphid-borne mosaic virus (CABMV) is a severe pathogen in peanut and a significant problem when distributing germ plasm to other countries. The virus is seedborne at approximately 0.15% in peanut, depending upon the cultivar, and its detection in seed lots would strengthen quarantine programs. Utilizing 3' sequence data (GenBank Accession #AF241233), primers were designed from the coat protein region and evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Use of the forward primer 5'-CGCTCAAACCCATTGTAGAA-3' and reverse primer 5'-TATTGCTTCCCTTGCTCTTTC-3' yielded a 221-bp product. Extracts of thick seed slices and a sample size of 12 to 25 seed showed no significant advantage of RT-PCR over enzyme-linked immunosorbent assay (ELISA) in tests of large seed lots. However, RT-PCR detected more virus in seed than in the number of infected seedlings normally arising in germination tests. Also, RT-PCR was extremely sensitive and detected 1 infected leaf among 99 healthy leaves. In contrast, ELISA detected only one infected leaf among nine healthy leaves
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|a Journal Article
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|a Pio-Ribeiro, G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Andrade, G P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pappu, H R
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 85(2001), 11 vom: 01. Nov., Seite 1181-1182
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|x 0191-2917
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|g volume:85
|g year:2001
|g number:11
|g day:01
|g month:11
|g pages:1181-1182
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|u http://dx.doi.org/10.1094/PDIS.2001.85.11.1181
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