Differentiation of Peanut Seedborne Potyviruses and Cucumoviruses by RT-PCR

Differentiation of Peanut Seedborne Potyviruses and Cucumoviruses by RT-PCR

Seedborne peanut viruses pose important constraints to peanut production and safe movement of germ plasm. They also pose a risk of accidental introduction into previously disease-free regions. We have developed reverse transcription-polymerase chain reaction (RT-PCR) assays based on identical cyclin...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Plant disease. - 1997. - 85(2001), 9 vom: 01. Sept., Seite 989-992
1. Verfasser: Dietzgen, Ralf G (VerfasserIn)
Weitere Verfasser: Callaghan, Ben, Higgins, Colleen M, Birch, Robert G, Chen, Kunrong, Xu, Zeyong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2001
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article duplex RT-PCR groundnut virus diagnosis
LEADER 01000naa a22002652 4500
001 NLM294474382
003 DE-627
005 20231225081546.0
007 cr uuu---uuuuu
008 231225s2001 xx |||||o 00| ||eng c
024 7 |a 10.1094/PDIS.2001.85.9.989  |2 doi 
028 5 2 |a pubmed24n0981.xml 
035 |a (DE-627)NLM294474382 
035 |a (NLM)30823115 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Dietzgen, Ralf G  |e verfasserin  |4 aut 
245 1 0 |a Differentiation of Peanut Seedborne Potyviruses and Cucumoviruses by RT-PCR 
264 1 |c 2001 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Revised 20.11.2019 
500 |a published: Print 
500 |a Citation Status PubMed-not-MEDLINE 
520 |a Seedborne peanut viruses pose important constraints to peanut production and safe movement of germ plasm. They also pose a risk of accidental introduction into previously disease-free regions. We have developed reverse transcription-polymerase chain reaction (RT-PCR) assays based on identical cycling parameters which identified peanut stripe, Peanut mottle, Peanut stunt, and Cucumber mosaic viruses through production of specific DNA fragments of 234 bp, 327 bp, 390 bp, and 133 bp, respectively. Assay sensitivity in the picogram range was achieved. The two potyviruses and two cucumoviruses could be differentiated using duplex RT-PCR assays. These assays should be useful for testing peanut leaves or seeds for virus identification in epidemiological studies, seed testing or in post-entry quarantine 
650 4 |a Journal Article 
650 4 |a duplex RT-PCR 
650 4 |a groundnut 
650 4 |a virus diagnosis 
700 1 |a Callaghan, Ben  |e verfasserin  |4 aut 
700 1 |a Higgins, Colleen M  |e verfasserin  |4 aut 
700 1 |a Birch, Robert G  |e verfasserin  |4 aut 
700 1 |a Chen, Kunrong  |e verfasserin  |4 aut 
700 1 |a Xu, Zeyong  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant disease  |d 1997  |g 85(2001), 9 vom: 01. Sept., Seite 989-992  |w (DE-627)NLM098181742  |x 0191-2917  |7 nnns 
773 1 8 |g volume:85  |g year:2001  |g number:9  |g day:01  |g month:09  |g pages:989-992 
856 4 0 |u http://dx.doi.org/10.1094/PDIS.2001.85.9.989  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 85  |j 2001  |e 9  |b 01  |c 09  |h 989-992