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231225s2001 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS.2001.85.8.856
|2 doi
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|a pubmed24n0981.xml
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|a (DE-627)NLM294473769
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|a (NLM)30823053
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Sether, D M
|e verfasserin
|4 aut
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| 245 |
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|a Differentiation, Distribution, and Elimination of Two Different Pineapple mealybug wilt-associated viruses Found in Pineapple
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|c 2001
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a Surveys for Pineapple mealybug wilt-associated virus-1 (PMWaV-1) and PMWaV-2 were conducted on pineapple samples from Hawaii and around the world. Tissue blot immunoassays (TBIAs) with two different monoclonal antibodies (MAb) specific to either PMWaV-1 or PMWaV-2 indicated that both closteroviruses are widely distributed throughout the pineapple-growing areas of the world. In the worldwide survey, PMWaV-1 was found in 80% of the mea-lybug wilt of pineapple (MWP)-symptomatic and 78% of the asymptomatic pineapple plants tested. A subset of plants was tested for PMWaV-2; 100% of the symptomatic plants and 12% of the asymptomatic plants were positive for this virus. A reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to differentiate between PMWaV-1 and PMWaV-2. Oligonucleotide primers were designed using distinct regions of the HSP 70 homolog genes of the two viruses. PMWaV-specific RT-PCR assays and TBIAs were used to screen the pineapple accessions maintained at the United States Department of Agriculture-Agricultural Research Service National Clonal Germplasm Repository for PMWaV infection; 73% of the accessions were found infected with at least one PMWaV. Pineapple accessions found PMWaV-free were challenged with viruliferous mealybugs to test for immunity to PMWaV-1. No immune germ plasm was identified. Potential alternative virus hosts were screened for infection with virus-specific RT-PCR assays and TBIAs and were also challenged with viruliferous mealybugs. No alternate hosts of PMWaV-1 or PMWaV-2 were identified. PMWaV-1 infection was eliminated through axillary and apical bud propagation from infected crowns. Strategies to manage MWP are discussed
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|a Journal Article
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|a spp.
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| 650 |
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4 |
|a MWP
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| 650 |
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4 |
|a PCV
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| 650 |
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4 |
|a closterovirus
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| 650 |
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4 |
|a mealybug wilt of pineapple
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| 700 |
1 |
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|a Karasev, A V
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Okumura, C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Arakawa, C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zee, F
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kislan, M M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Busto, J L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hu, J S
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 85(2001), 8 vom: 01. Aug., Seite 856-864
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
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|g volume:85
|g year:2001
|g number:8
|g day:01
|g month:08
|g pages:856-864
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| 856 |
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|u http://dx.doi.org/10.1094/PDIS.2001.85.8.856
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