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|a 10.1094/PDIS.2002.86.3.328B
|2 doi
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|a eng
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| 100 |
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|a Khan, J A
|e verfasserin
|4 aut
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|a The Association of Begomovirus with Bitter Melon in India
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|c 2002
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a Bitter melon, Momordica charantia (Cucurbitaceae), is a vegetable of nutritive and medicinal value that is cultivated throughout India and other tropical countries. In September 2001, a severe disease of bitter melon with virus-like symptoms was observed at Lucknow, India. Symptoms consisted of upward curling, shortening, and distortion of leaves. Diseased melon fruits were stunted and deformed. Disease incidence was as high as 100%. Whitefly (Bemicia tabaci) can transmit the associated virus from diseased bitter melon to Nicotiana tabacum cv. White burley. The development of leaf curl symptoms in N. tabacum indicated the pathogen could be a begomovirus. Total nucleic acids were extracted from diseased bitter melon leaves, and polymerase chain reaction (PCR) tests were performed. Three pairs of primers, AV494 and AC1048 (1), CL-CR/F2 and CL-CR/R2, CL/11F and CL10/R (2), specific to DNA-A of begomoviruses were used in PCR. Virus-specific DNA-A fragments of expected sizes were identified (≈0.5, 0.7 and 1.2 kb, respectively). The presence of a begomovirus in all PCR-amplified DNA fragments was confirmed by Southern hybridization. Cloned DNA-A fragments of Tomato leaf curl virus and Cotton leaf curl virus (both begomoviruses) cross-hybridized with the PCR products gave strong signals under high stringency conditions. These data suggest that a begomovirus is associated with this bitter melon disease. Watermelon mosaic 1 virus is the only virus previously reported to naturally infect bitter melon; however, this virus has not been identified in India. Bitter melon is also an experimental host of Ribgrass mosaic virus (genus Tobamovirus) and Trichosanthes mottle virus (genus Potyvirus). To our knowledge, this is the first report of the occurrence of begomovirus infecting bitter melon. References: (1) S. D. Wyatt and J. K. Brown. Phytopathology 86:1288, 1996. (2). X. Zhou et al. J. Gen. Virol. 79:915, 1998
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|a Journal Article
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|a Siddiqui, M K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Singh, B P
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 86(2002), 3 vom: 01. März, Seite 328
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|x 0191-2917
|7 nnns
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| 773 |
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|g volume:86
|g year:2002
|g number:3
|g day:01
|g month:03
|g pages:328
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|u http://dx.doi.org/10.1094/PDIS.2002.86.3.328B
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