Rapid extraction of DNA suitable for NGS workflows from bacterial cultures using the PDQeX
Background: PDQeX is a novel, single-step DNA extraction method that purifies nucleic acid from sample in under 30 min. Materials & Methods: Six bacterial suspensions from species with different cell morphologies and growth optima were made. DNA from half the suspension was purified using PDQeX...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 66(2019), 5 vom: 01. Mai, Seite 208-213
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| 1. Verfasser: |
Stanton, Jo-Ann L
(VerfasserIn) |
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| Weitere Verfasser: |
Muralidhar, Abishek,
Rand, Christy J,
Saul, David J |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2019
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
DNA extraction
Ion PGM
PDQeX
QIAGEN DNeasy
ZyGEM
bacterial DNA preparation
genome sequencing
whole-genome sequencing
DNA, Bacterial |
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