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231225s2003 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS.2003.87.7.872B
|2 doi
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|a DE-627
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|a eng
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| 100 |
1 |
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|a Verhoeven, J Th J
|e verfasserin
|4 aut
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| 245 |
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|a First Report of Tomato infectious chlorosis virus in Tomato in Indonesia
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|c 2003
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|a Text
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|a ƒaComputermedien
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a In 2002, a breeding company submitted several samples of tomato (Lycopersicon esculentum) for diagnosis. Samples originated in Indonesia and were taken from protected and nonprotected crops. Plants exhibited severe chlorosis on fully expanded leaves, while young leaves were symptomless. Symptoms resembled those of the criniviruses Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV). Moreover, large numbers of whiteflies, potential vectors of these viruses, had been observed at the plots with symptomatic plants. A reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for TICV (1) yielded amplicons of the expected size of approximately 500 bp for all samples. One of the amplicons was sequenced (Genbank Accession No. AY221097) and revealed more than 98.9% identity to six isolates of TICV in NCBI Genbank. cDNA synthesis using the universal crinivirus primer HSP_M2-DW (5' -TCRAARGTWCCKCCNCCRAA-3') followed by PCR with a ToCV specific primerset (ToCV-UP 5'-TCATTAAAACTCAATGGGACCGAG-3' and ToCV-DW 5'-GCGACGT AAATTGAAACCC-3') was negative in all cases. Grafting of symptomatic shoots onto healthy tomato seedlings of cv. Money-maker showed transmission of the virus, as chlorosis appeared on fully expanded leaves of lateral shoots after 6 weeks. The presence of TICV in the graft-inoculated plants was confirmed by RT-PCR. Furthermore, mechanical inoculation to a range of herbaceous test plants did not evoke any virus symptoms, indicating the absence of mechanically transmissible viruses. Although other nonmechanically transmissible viruses cannot be fully excluded, the results together with the symptoms observed, indicate that TICV is the cause of the disease. TICV has been reported from Greece, Italy, Japan, Spain, and the United States, but to our knowledge, this is the first report of TICV in Indonesia. Reference: (1) A. M. Vaira et al. Phytoparasitica 30:290, 2002
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|a Journal Article
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1 |
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|a Willemen, T M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Roenhorst, J W
|e verfasserin
|4 aut
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| 700 |
1 |
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|a van der Vlugt, R A A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 87(2003), 7 vom: 20. Juli, Seite 872
|w (DE-627)NLM098181742
|x 0191-2917
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| 773 |
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|g volume:87
|g year:2003
|g number:7
|g day:20
|g month:07
|g pages:872
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|u http://dx.doi.org/10.1094/PDIS.2003.87.7.872B
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