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231225s2003 xx |||||o 00| ||eng c |
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7 |
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|a 10.1094/PDIS.2003.87.1.33
|2 doi
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|a pubmed24n0981.xml
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|a eng
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| 100 |
1 |
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|a Delanoy, M
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Development of Real-Time PCR for the Rapid Detection of Episomal Banana streak virus (BSV)
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| 264 |
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|c 2003
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a A real-time assay for the detection of episomal Banana streak virus (BSV; strain OL) in banana and plantains that carry integrated BSV sequences is described. Primers specific to the viral DNA were designed using the viral sequence integrated into the cv. Obino l'Ewai genome and the sequence of the genomic DNA of the infecting virus strain OL. They amplify a sequence of 1,336 bp that is detected in real-time by a short fluorogenic 3' minor groove binder DNA probe. This method enables reproducible and specific detection of episomal BSV from purified DNA as well as from crude extracts from infected plants. The assay is rapid, adaptable for large-scale experiments, and circumvents carryover problems
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|a Journal Article
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| 650 |
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4 |
|a 5′ nuclease assay
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| 650 |
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4 |
|a badnavirus
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| 650 |
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|a long amplicon
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| 650 |
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4 |
|a minor groove binder (MGB)
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| 700 |
1 |
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|a Salmon, M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kummert, J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Frison, E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lepoivre, P
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 87(2003), 1 vom: 20. Jan., Seite 33-38
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
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|g volume:87
|g year:2003
|g number:1
|g day:20
|g month:01
|g pages:33-38
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| 856 |
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|u http://dx.doi.org/10.1094/PDIS.2003.87.1.33
|3 Volltext
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|d 87
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|h 33-38
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