Engineered Bacterial Bioreactor for Tumor Therapy via Fenton-Like Reaction with Localized H2 O2 Generation

Bibliographische Detailangaben
Veröffentlicht in:	Advanced materials (Deerfield Beach, Fla.). - 1998. - 31(2019), 16 vom: 01. Apr., Seite e1808278
1. Verfasser:	Fan, Jin-Xuan (VerfasserIn)
Weitere Verfasser:	Peng, Meng-Yun, Wang, He, Zheng, Hao-Ran, Liu, Zong-Lin, Li, Chu-Xin, Wang, Xia-Nan, Liu, Xin-Hua, Cheng, Si-Xue, Zhang, Xian-Zheng
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2019
Zugriff auf das übergeordnete Werk:	Advanced materials (Deerfield Beach, Fla.)
Schlagworte:	Journal Article Fenton-like reaction bacterial bioreactor chemodynamic therapy synthetic biology tumor therapy Magnetite Nanoparticles Reactive Oxygen Species Hydroxyl Radical 3352-57-6 mehr... Hydrogen Peroxide BBX060AN9V

Beschreibung
Zusammenfassung:	© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Synthetic biology based on bacteria has been displayed in antitumor therapy and shown good performance. In this study, an engineered bacterium Escherichia coli MG1655 is designed with NDH-2 enzyme (respiratory chain enzyme II) overexpression (Ec-pE), which can colonize in tumor regions and increase localized H2 O2 generation. Following from this, magnetic Fe3 O4 nanoparticles are covalently linked to bacteria to act as a catalyst for a Fenton-like reaction, which converts H2 O2 to toxic hydroxyl radicals (•OH) for tumor therapy. In this constructed bioreactor, the Fenton-like reaction occurs with sustainably synthesized H2 O2 produced by engineered bacteria, and severe tumor apoptosis is induced via the produced toxic •OH. These results show that this bioreactor can achieve effective tumor colonization, and realize a self-supplied therapeutic Fenton-like reaction without additional H2 O2 provision
Beschreibung:	Date Completed 08.08.2019 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-4095
DOI:	10.1002/adma.201808278