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231225s2006 xx |||||o 00| ||eng c |
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|a 10.1094/PD-90-0723
|2 doi
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5 |
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|a pubmed24n0980.xml
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|a DE-627
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Vaïanopoulos, C
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Widespread Occurrence of Wheat spindle streak mosaic virus in Belgium
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| 264 |
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1 |
|c 2006
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Revised 20.11.2019
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| 500 |
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a In order to assess the occurrence of Wheat spindle streak mosaic virus (WSSMV) in Belgium, a reverse-transcription polymerase chain reaction (RT-PCR) was developed, targeting WSSMV isolates from Canada, France, Germany, Italy, and the United States. The primers also were designed for virus quantification by real-time RT-PCR with SYBR-Green. No cross-reaction with soilborne cereal viruses such as Barley mild mosaic virus, Barley yellow mosaic virus, Soilborne cereal mosaic virus, and Soil-borne wheat mosaic virus was observed. The RT-PCR and real-time quantitative RT-PCR allowed a more sensitive detection of WSSMV than enzymelinked immunosorbent assay. The incidence of WSSMV in Belgium was evaluated using a bioassay with wheat cvs. Cezanne and Savannah and rye cv. Halo, grown in 104 Belgian soils. The presence of WSSMV was detected from plants grown in 32% of the soils. The RT-PCR methods developed here, combined with large sampling, allowed WSSMV to be detected for the first time in Belgium. The real-time quantitative RT-PCR was developed as a tool for evaluating the resistance to WSSMV by quantifying the virus concentration in wheat cultivars
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a soilborne viruses
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| 700 |
1 |
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|a Legrève, A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lorca, C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Moreau, V
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Steyer, S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Maraite, H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bragard, C
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 90(2006), 6 vom: 01. Juni, Seite 723-728
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
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|g volume:90
|g year:2006
|g number:6
|g day:01
|g month:06
|g pages:723-728
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| 856 |
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|u http://dx.doi.org/10.1094/PD-90-0723
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