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231225s2006 xx |||||o 00| ||eng c |
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|a 10.1094/PD-90-1395
|2 doi
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|a pubmed24n0980.xml
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Xue, H Q
|e verfasserin
|4 aut
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| 245 |
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|a Ergosterol as a Quantifiable Biomass Marker for Diaporthe haseolorum and Cercospora kikuchi
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| 264 |
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|c 2006
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a The relationship between ergosterol content and biomass was determined for the soybean fungal pathogens Diaporthe phaseolorum (Cooke & Ellis) Sacc. var. sojae, causal agent of Phomopsis seed decay, and Cercospora kikuchii (Matsumoto & Tomoy.), causal agent of leaf blight and purple seed stain. Biomass was manipulated by varying incubation period, and ergosterol was quantified by high-pressure liquid chromatography. Fungal dry mass was linearly correlated with ergosterol content (r2 = 0.90, P < 0.05 for D. phaseolorum, and r2 = 0.95, P < 0.01 for C. kikuchii). In vitro ergosterol content of fungi was 3.16 μg/mg for D. phaseolorum and 2.85 μg/mg for C. kikuchii. Ergosterol content of inoculated seed was qualitatively correlated with observed seed colonization by both pathogens. Soybean variety had a significant effect on fungal colonization by D. phaseolorum and ergosterol content. Results show that ergosterol content can be used to quantify colonization of soybean seed by both pathogens
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| 650 |
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|a Journal Article
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1 |
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|a Upchurch, R G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kwanyuen, P
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 90(2006), 11 vom: 01. Nov., Seite 1395-1398
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
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|g volume:90
|g year:2006
|g number:11
|g day:01
|g month:11
|g pages:1395-1398
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|u http://dx.doi.org/10.1094/PD-90-1395
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|d 90
|j 2006
|e 11
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|c 11
|h 1395-1398
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