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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erz059
|2 doi
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|a pubmed24n0980.xml
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|a (DE-627)NLM294038825
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|a (NLM)30778561
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Soares, André
|e verfasserin
|4 aut
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|a An atypical aspartic protease modulates lateral root development in Arabidopsis thaliana
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 15.06.2020
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|a Date Revised 14.07.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Few atypical aspartic proteases (APs) present in plants have been functionally studied to date despite having been implicated in developmental processes and stress responses. Here we characterize a novel atypical AP that we name Atypical Aspartic Protease in Roots 1 (ASPR1), denoting its expression in Arabidopsis roots. Recombinant ASPR1 produced by transient expression in Nicotiana benthamiana was active and displayed atypical properties, combining optimum acidic pH, partial sensitivity to pepstatin, pronounced sensitivity to redox agents, and unique specificity preferences resembling those of fungal APs. ASPR1 overexpression suppressed primary root growth and lateral root development, implying a previously unknown biological role for an AP. Quantitative comparison of wild-type and aspr1 root proteomes revealed deregulation of proteins associated with both reactive oxygen species and auxin homeostasis in the mutant. Together, our findings on ASPR1 reinforce the diverse pattern of enzymatic properties and biological roles of atypical APs and raise exciting questions on how these distinctive features impact functional specialization among these proteases
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis thaliana
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|a ASPR1
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|a atypical aspartic protease
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|a auxin
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|a lateral root
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|a magnICON expression system
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|a reactive oxygen species
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|a root development
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|a Arabidopsis Proteins
|2 NLM
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|a AT2G03200 protein, Arabidopsis
|2 NLM
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| 650 |
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|a EC 3.4.-
|2 NLM
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| 650 |
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|a Aspartic Acid Proteases
|2 NLM
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| 650 |
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|a EC 3.4.-
|2 NLM
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| 700 |
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|a Niedermaier, Stefan
|e verfasserin
|4 aut
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| 700 |
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|a Faro, Rosário
|e verfasserin
|4 aut
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| 700 |
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|a Loos, Andreas
|e verfasserin
|4 aut
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| 700 |
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|a Manadas, Bruno
|e verfasserin
|4 aut
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| 700 |
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|a Faro, Carlos
|e verfasserin
|4 aut
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| 700 |
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|a Huesgen, Pitter F
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheung, Alice Y
|e verfasserin
|4 aut
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| 700 |
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|a Simões, Isaura
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 70(2019), 7 vom: 12. Apr., Seite 2157-2171
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:70
|g year:2019
|g number:7
|g day:12
|g month:04
|g pages:2157-2171
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erz059
|3 Volltext
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