Detection and Discrimination of Pratylenchus neglectus and P. thornei in DNA Extracts from Soil
A species-specific polymerase chain reaction (PCR) method was developed to detect and identify the root-lesion nematodes Pratylenchus neglectus and P. thornei from soil. A primer set was designed from Pratylenchus 28S rRNA gene sequences of the D3 expansion domain. Primer specificity was confirmed w...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | Plant disease. - 1997. - 92(2008), 11 vom: 11. Nov., Seite 1480-1487
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| 1. Verfasser: |
Yan, Guiping
(VerfasserIn) |
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| Weitere Verfasser: |
Smiley, Richard W,
Okubara, Patricia A,
Skantar, Andrea,
Easley, Sandra A,
Sheedy, Jason G,
Thompson, Alison L |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | Plant disease
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| Schlagworte: | Journal Article
DNA extraction
DNA purification
detection sensitivity |
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