Development of a Nested Polymerase Chain Reaction Assay for Detection of Colletotrichum acutatum on Symptomless Strawberry Leaves

A nested polymerase chain reaction (PCR) assay was developed for detection of Colletotrichum acutatum on symptomless strawberry leaves. In pure culture, the assay detected as little as 1.0 fg of DNA extracted from mycelium and as few as 1.5 conidia ml-1 when conidial suspensions were sonicated. On d...

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Veröffentlicht in:	Plant disease. - 1997. - 92(2008), 12 vom: 11. Dez., Seite 1655-1661
1. Verfasser:	Pérez-Hernández, O (VerfasserIn)
Weitere Verfasser:	Nam, M H, Gleason, M L, Kim, H G
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2008
Zugriff auf das übergeordnete Werk:	Plant disease
Schlagworte:	Journal Article DNA extraction anthracnose