Development of a Nested Polymerase Chain Reaction Assay for Detection of Colletotrichum acutatum on Symptomless Strawberry Leaves

A nested polymerase chain reaction (PCR) assay was developed for detection of Colletotrichum acutatum on symptomless strawberry leaves. In pure culture, the assay detected as little as 1.0 fg of DNA extracted from mycelium and as few as 1.5 conidia ml-1 when conidial suspensions were sonicated. On d...

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Détails bibliographiques

Publié dans:	Plant disease. - 1997. - 92(2008), 12 vom: 11. Dez., Seite 1655-1661
Auteur principal:	Pérez-Hernández, O (Auteur)
Autres auteurs:	Nam, M H, Gleason, M L, Kim, H G
Format:	Article en ligne
Langue:	English
Publié:	2008
Accès à la collection:	Plant disease
Sujets:	Journal Article DNA extraction anthracnose