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231225s2009 xx |||||o 00| ||eng c |
| 024 |
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|a 10.1094/PDIS-93-5-0499
|2 doi
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|a pubmed24n0979.xml
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|a (NLM)30764134
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|a DE-627
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Nikolaeva, Ekaterina V
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Ratios of Cells With and Without Virulence Genes in Rhodococcus fascians Populations Correlate with Degrees of Symptom Development
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| 264 |
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1 |
|c 2009
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Revised 20.11.2019
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a Rhodococcus fascians, a gram-positive phytopathogenic bacterium, causes fasciation and leafy galls on a wide range of monocotyledonous and dicotyledonous plants for which it requires the plasmid-borne fas operon. Strains isolated from symptomatic plants over a 20-year-period exhibited a high degree of variability when their virulence was assessed on garden pea seedlings. Polymerase chain reaction amplification of the fas-1 and fasR virulence genes from randomly chosen single colonies showed that many strains consisted of two subpopulations, of which one had lost these genes. Inoculation of pea seedlings with mixtures of fas-1-positive and -negative cells that originated from the same strain demonstrated a strong correlation (Pearson's r ≥ 0.9205) between the proportion of cells in the inoculum carrying the fas-1 gene and the severity of disease symptoms. The minimal concentration of fas-1-positive cells required for the development of small lateral shoots on pea seedlings was 2.5 × 104 CFU/ml (P ≤ 0.008), while the overall suppression of main stem growth was observed at 2.5 × 105 CFU/ml (P ≤ 0.019). These observations underline that care should be taken when the virulence of clinical R. fascians strains is evaluated
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| 650 |
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4 |
|a Journal Article
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| 700 |
1 |
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|a Park, Sook-Young
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kang, Seogchan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Olson, Tracey N
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, Seong H
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 93(2009), 5 vom: 11. Mai, Seite 499-506
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
8 |
|g volume:93
|g year:2009
|g number:5
|g day:11
|g month:05
|g pages:499-506
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| 856 |
4 |
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|u http://dx.doi.org/10.1094/PDIS-93-5-0499
|3 Volltext
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| 952 |
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|d 93
|j 2009
|e 5
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|h 499-506
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