Amelioration of nitrate uptake under salt stress by ectomycorrhiza with and without a Hartig net

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1984. - 222(2019), 4 vom: 12. Juni, Seite 1951-1964
1. Verfasser:	Sa, Gang (VerfasserIn)
Weitere Verfasser:	Yao, Jun, Deng, Chen, Liu, Jian, Zhang, Yinan, Zhu, Zhimei, Zhang, Yuhong, Ma, Xujun, Zhao, Rui, Lin, Shanzhi, Lu, Cunfu, Polle, Andrea, Chen, Shaoliang
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2019
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Paxillus involutus MAJ NAU NO3− flux NRTs NaCl Poplar pH mehr... Membrane Transport Proteins Nitrates Vanadates 3WHH0066W5 Sodium Chloride 451W47IQ8X Nitrite Reductases EC 1.7.- Nitrate Reductase EC 1.7.99.4 Proton-Translocating ATPases EC 3.6.3.14

Beschreibung
Zusammenfassung:	© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust. Salt stress is an important environmental cue impeding poplar nitrogen nutrition. Here, we characterized the impact of salinity on proton-driven nitrate fluxes in ectomycorrhizal roots and the importance of a Hartig net for nitrate uptake. We employed two Paxillus involutus strains for root colonization: MAJ, which forms typical ectomycorrhizal structures (mantle and Hartig net), and NAU, colonizing roots with a thin, loose hyphal sheath. Fungus-colonized and noncolonized Populus × canescens were exposed to sodium chloride and used to measure root surface pH, nitrate (NO3- ) flux and transcription of NO3- transporters (NRTs; PcNRT1.1, -1.2, -2.1), and plasmalemma proton ATPases (HAs; PcHA4, -8, -11). Paxillus colonization enhanced root NO3- uptake, decreased surface pH, and stimulated NRTs and HA4 of the host regardless the presence or absence of a Hartig net. Under salt stress, noncolonized roots exhibited strong net NO3- efflux, whereas beneficial effects of fungal colonization on surface pH and HAs prevented NO3- loss. Inhibition of HAs abolished NO3- influx under all conditions. We found that stimulation of HAs was crucial for the beneficial influence of ectomycorrhiza on NO3- uptake, whereas the presence of a Hartig net was not required for improved NO3- translocation. Mycorrhizas may contribute to host adaptation to salt-affected environments by keeping up NO3- nutrition
Beschreibung:	Date Completed 27.02.2020 Date Revised 12.10.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.15740