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231225s2010 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-94-2-0207
|2 doi
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|a eng
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| 100 |
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|a Young, Joseph R
|e verfasserin
|4 aut
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|a Two Mutations in β-Tubulin 2 Gene Associated with Thiophanate-Methyl Resistance in Colletotrichum cereale Isolates from Creeping Bentgrass in Mississippi and Alabama
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|c 2010
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a Turfgrass anthracnose, caused by Colletotrichum cereale (≡C. graminicola), has become a common disease of creeping bentgrass putting greens during the summer in Mississippi and Alabama over the last 15 years. Thiophanate-methyl is a single-site mode-of-action fungicide applied to control C. cereale. In vitro bioassays were performed to evaluate the sensitivity of 103 isolates to thiophanate-methyl concentrations ranging from 0.039 to 10 μg/ml. Eighty-three isolates were collected from creeping bentgrass in Mississippi and Alabama that had been exposed to thiophanate-methyl. An additional 20 isolates were included from nonexposed turfgrasses. Radial colony growth in amended media was relative to nonamended media for all in vitro bioassays. With thiophanate-methyl at 10 μg/ml, relative growth of exposed isolates ranged from 77.5 to 130.7% with a mean of 99.3% compared with nonexposed, baseline isolates that ranged from 0.0 to 48.7% with a mean of 20.4%. A representative sample of thiophanate-methyl-exposed and nonexposed isolates was used to determine the mechanism of resistance by comparing amino acid sequences of the β-tubulin 2 protein. All of the thiophanate-methyl-exposed isolates that were sequenced had a point mutation resulting in substitutions from glutamic acid to alanine at position 198 or from phenylalanine to tyrosine at position 200 of the β-tubulin 2 protein. These amino acid substitutions in C. cereale isolates from Mississippi and Alabama appear to confer resistance to thiophanate-methyl and differ from those reported previously for this pathogen
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|a Journal Article
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1 |
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|a Tomaso-Peterson, Maria
|e verfasserin
|4 aut
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| 700 |
1 |
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|a de la Cerda, Karla
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wong, Francis P
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 94(2010), 2 vom: 13. Feb., Seite 207-212
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnas
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| 773 |
1 |
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|g volume:94
|g year:2010
|g number:2
|g day:13
|g month:02
|g pages:207-212
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| 856 |
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|u http://dx.doi.org/10.1094/PDIS-94-2-0207
|3 Volltext
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|d 94
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